Genetic diversity of Mycobacterium tuberculosis isolates from Tochigi prefecture, a local region of Japan

BMC Infectious Diseases

Research Article

Hiroki Iwai¹  Tohru Miyoshi-Akiyama²  Teruo Kirikae³  Fuminori Mizukoshi⁴  Takako Suzuki⁴  Keiji Funatogawa⁴  Reiko Kiritani⁴ 

[1] Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan;Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan;Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan;Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan;Present Address: Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan;Tochigi Prefectural Institute of Public Health and Environmental Science, Utsunomiya, Tochigi, Japan;

关键词: Foreign-born;    Lineage;    Whole genome sequencing;    CASTB;    Japan;   

DOI  :  10.1186/s12879-017-2457-y

 received in 2016-11-07, accepted in 2017-05-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundForeign-born patients with tuberculosis (TB) may introduce globally disseminated isolates of Mycobacterium tuberculosis into large cities in Japan. The risk of dissemination of these isolates into local regions, however, has not been determined. This study analyzed the molecular epidemiology of M. tuberculosis isolates obtained from TB patients living in a local region of Japan.MethodsWhole genome sequences of 169 M. tuberculosis isolates, obtained from 148 Japanese-born and 21 foreign-born patients living in Tochigi, Japan, were analyzed using the Comprehensive analysis server for the Mycobacteriumtuberculosis complex (CASTB).ResultsThe 169 isolates were clustered into four clades; Lineage 2 (111 isolates 65.7%), Lineage 4 (43 isolates, 25.4%), Lineage 1 (13 isolates, 7.7%), and Lineage 3 (2 isolates, 1.2%). Of the 111 isolates belonging to Lineage 2, 79 (71.2%) were of the atypical Beijing sub-genotype. Of the 13 Lineage 1 isolates, nine (69.2%) were from foreign-born patients. The isolates belonging to Lineage 4 were further clustered into three clades, two containing isolates shared by both Japanese- and foreign-born patients. The two isolates belonging to Lineage 3 were obtained from foreign-born patients.ConclusionsThe genotypic diversity of M. tuberculosis in a local region of Japan is increased primarily by the presence of isolates obtained from foreign-born patients.

【 授权许可】

CC BY   
© The Author(s). 2017