Opportunities for improving pLDH-based malaria diagnostic tests

Malaria Journal

Methodology

Young Ho Choi¹  Robert C Piper²  Michael T Makler²  Ian Buchanan³ 

[1] Access Bio, Inc. 65 Clyde Rd. Suite A Somerset, 0887, NJ, USA;Flow Incorporated, 6127 SW Corbett, 97239-3601, Portland, OR, USA;Vista Diagnostics, 11410 124th NE St, 98034, 484 Kirkland, WA, USA;

关键词: Malaria;    Colloidal Gold;    Test Strip;    Rapid Test;    Immunochromatographic Assay;   

DOI  :  10.1186/1475-2875-10-213

 received in 2011-03-24, accepted in 2011-08-01,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMonoclonal antibodies to Plasmodium lactate dehydrogenase (pLDH) have been previously used to format immunochromatographic tests for the diagnosis of malaria. Using pLDH as an antigen has several advantages as a sensitive measure of the presence of parasites within patient blood samples. However, variable results in terms of specificity and sensitivity among different commercially available diagnostic kits have been reported and it has not been clear from these studies whether the performance of an individual test is due simply to how it is engineered or whether it is due to the biochemical nature of the pLDH-antibody reaction itself.MethodsA series of systematic studies to determine how various pLDH monoclonal antibodies work in combination was undertaken. Different combinations of anti-pLDH monoclonal antibodies were used in a rapid-test immunochromatographic assay format to determine parameters of sensitivity and specificity with regard to individual Plasmodium species.ResultsDramatic differences were found in both species specificity and overall sensitivity depending on which antibody is used on the immunochromatographic strip and which is used on the colorimetric colloidal-gold used for visual detection.DiscussionThe results demonstrate the feasibility of different test formats for the detection and speciation of malarial infections. In addition, the data will enable the development of a universal rapid test algorithm that may potentially provide a cost-effective strategy to diagnose and manage patients in a wide range of clinical settings.ConclusionThese data emphasize that using different anti-pLDH antibody combinations offers a tractable way to optimize immunochromatographic pLDH tests.

【 授权许可】

CC BY   
© Piper et al; licensee BioMed Central Ltd. 2011