Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)

BMC Genomics

Research Article

Kathryn Lilley¹  Lee A Meisel²  Ricardo Nilo²  Carlos Saffie²  Ariel Orellana²  Reinaldo Campos-Vargas³  Ricardo Baeza-Yates⁴  Julio Retamales⁵  Mauricio González⁶  Verónica Cambiazo⁷  Herman Silva⁸ 

[1] Cambridge Centre for Proteomics, University of Cambridge, Cambridge, UK;Centro de Biotecnología Vegetal, Universidad Andrés Bello, Santiago, Chile;Millennium Nucleus in Plant Cell Biotechnology (MN-PCB), Santiago, Chile;Centro de Biotecnología Vegetal, Universidad Andrés Bello, Santiago, Chile;Millennium Nucleus in Plant Cell Biotechnology (MN-PCB), Santiago, Chile;Institute of Agricultural Research (INIA-La Platina), Santiago, Chile;Dept. of Computer Science, Universidad de Chile, Santiago, Chile;Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago, Chile;Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile, Santiago, Chile;Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile, Santiago, Chile;Millennium Nucleus Center for Genomics of the Cell (CGC), Santiago, Chile;Millennium Nucleus in Plant Cell Biotechnology (MN-PCB), Santiago, Chile;Plant Functional Genomics & Bioinformatics Lab, Universidad Andrés Bello, Santiago, Chile;

关键词: Cold Storage;    Ripe Fruit;    Small Heat Shock Protein;    Chilling Injury;    Fruit Softening;   

DOI  :  10.1186/1471-2164-11-43

 received in 2009-05-25, accepted in 2010-01-18,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPeach fruit undergoes a rapid softening process that involves a number of metabolic changes. Storing fruit at low temperatures has been widely used to extend its postharvest life. However, this leads to undesired changes, such as mealiness and browning, which affect the quality of the fruit. In this study, a 2-D DIGE approach was designed to screen for differentially accumulated proteins in peach fruit during normal softening as well as under conditions that led to fruit chilling injury.ResultsThe analysis allowed us to identify 43 spots -representing about 18% of the total number analyzed- that show statistically significant changes. Thirty-nine of the proteins could be identified by mass spectrometry. Some of the proteins that changed during postharvest had been related to peach fruit ripening and cold stress in the past. However, we identified other proteins that had not been linked to these processes. A graphical display of the relationship between the differentially accumulated proteins was obtained using pairwise average-linkage cluster analysis and principal component analysis. Proteins such as endopolygalacturonase, catalase, NADP-dependent isocitrate dehydrogenase, pectin methylesterase and dehydrins were found to be very important for distinguishing between healthy and chill injured fruit. A categorization of the differentially accumulated proteins was performed using Gene Ontology annotation. The results showed that the 'response to stress', 'cellular homeostasis', 'metabolism of carbohydrates' and 'amino acid metabolism' biological processes were affected the most during the postharvest.ConclusionsUsing a comparative proteomic approach with 2-D DIGE allowed us to identify proteins that showed stage-specific changes in their accumulation pattern. Several proteins that are related to response to stress, cellular homeostasis, cellular component organization and carbohydrate metabolism were detected as being differentially accumulated. Finally, a significant proportion of the proteins identified had not been associated with softening, cold storage or chilling injury-altered fruit before; thus, comparative proteomics has proven to be a valuable tool for understanding fruit softening and postharvest.

【 授权许可】

Unknown   
© Nilo et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.