Detection of Lipid-Rich Prostate Circulating Tumour Cells with Coherent Anti-Stokes Raman Scattering Microscopy

BMC Cancer

Research Article

Yasuyo Urasaki¹  Thuc T Le¹  Olivia Chao²  Oscar B Goodman²  Ranjana Mitra² 

[1] Nevada Cancer Institute, One Breakthrough Way, 89135, Las Vegas, NV, USA;Desert Research Institute, 10530 Discovery Drive, 89135, Las Vegas, NV, USA;Nevada Cancer Institute, One Breakthrough Way, 89135, Las Vegas, NV, USA;Roseman University of Health Sciences, 11 Sunset Way, 89014, Henderson, NV, USA;

关键词: Circulate Tumour Cell;    Metastatic Prostate Cancer;    Intracellular Lipid;    Circulate Tumour Cell Detection;    Metastatic Prostate Cancer Cell;   

DOI  :  10.1186/1471-2407-12-540

 received in 2012-08-08, accepted in 2012-10-11,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCirculating tumour cells (CTC) are an important indicator of metastasis and associated with a poor prognosis. Detection sensitivity and specificity of CTC in the peripheral blood of metastatic cancer patient remain a technical challenge.MethodsCoherent anti-Stokes Raman scattering (CARS) microscopy was employed to examine the lipid content of CTC isolated from the peripheral blood of metastatic prostate cancer patients. CARS microscopy was also employed to evaluate lipid uptake and mobilization kinetics of a metastatic human prostate cancer cell line.ResultsOne hundred CTC from eight metastatic prostate cancer patients exhibited strong CARS signal which arose from intracellular lipid. In contrast, leukocytes exhibited weak CARS signal which arose mostly from cellular membrane. On average, CARS signal intensity of prostate CTC was 7-fold higher than that of leukocytes (P<0.0000001). When incubated with human plasma, C4-2 metastatic human prostate cancer cells exhibited rapid lipid uptake kinetics and slow lipid mobilization kinetics. Higher expression of lipid transport proteins in C4-2 cells compared to non-transformed RWPE-1 and non-malignant BPH-1 prostate epithelial cells further indicated strong affinity for lipid of metastatic prostate cancer cells.ConclusionsIntracellular lipid could serve as a biomarker for prostate CTC which could be sensitively detected with CARS microscopy in a label-free manner. Strong affinity for lipid by metastatic prostate cancer cells could be used to improve detection sensitivity and therapeutic targeting of prostate CTC.

【 授权许可】

Unknown   
© Mitra et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.