Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae

Microbial Cell Factories

Research

Xianzhen Li¹  Xue Wang¹  Zhi-Min Yu¹  Lan Yang¹  Fan Yang¹  Zhi-Cheng Liu¹  Li-Li Li¹  Wen-Zhu Tang¹ 

[1] School of Biological Engineering, Dalian Polytechnic University, 116034, Dalian, China;

关键词: Invertase;    Transcript level;    Saccharomyces cerevisiae;    Inulin;   

DOI  :  10.1186/s12934-015-0243-3

 received in 2014-07-11, accepted in 2015-04-08,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundInvertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate how Suc2 activity is regulated in S. cerevisiae.ResultsThe effect of SUC2 expression level on inulin hydrolysis was investigated by introducing different SUC2 genes or their corresponding promoters in S. cerevisiae strain BY4741 that can only weakly catabolize inulin. Both inulinase and invertase activities were increased with the rising SUC2 expression level. Variation in the promoter sequence has an obvious effect on the transcript level of the SUC2 gene. It was also found that the high expression level of SUC2 was beneficial to inulin degradation and ethanol yield.ConclusionsSuc2-mediated inulin catabolism is regulated at transcript level in S. cerevisiae. Our work should be valuable for engineering advanced yeast strains in application of inulin for ethanol production.

【 授权许可】

Unknown   
© Yang et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.