The response of canine faecal microbiota to increased dietary protein is influenced by body condition

BMC Veterinary Research

Research Article

Miguel Campos¹  Myriam Hesta²  Daisy J. X. Liu²  An Cools²  Marta Lourenço²  Jia Xu³  Adronie Verbrugghe⁴  Filip Van Immerseel⁵  Venessa Eeckhaut⁵  Lynn Vanhaecke⁶  Massimo Marzorati⁷  Tom Van de Wiele⁷ 

[1] Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium;Department of Clinical Veterinary Science, Vetsuisse Faculty, Bern University, Länggassstrasse 128, 3001, Bern, Switzerland;Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium;Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium;Present Address: Department of Agriculture and Bioengineering, Jinhua Polytechnic, Wuzhou steet 1188, 321007, Jinhua, Zhejiang Province, People’s Republic of China;Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium;Present Address: Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road E, N1G 2W1, Guelph, ON, Canada;Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium;Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium;Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium;

关键词: Butyrate-producing bacteria;    Butyrate kinase;    Canine;    Obesity;    Protein fermentation;   

DOI  :  10.1186/s12917-017-1276-0

 received in 2017-01-02, accepted in 2017-11-16,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundHigh protein diets shift the faecal microbiota into a more unfavourable composition in obese humans. In lean dogs, higher protein consumption is accompanied with increased production of putrefactive fermentation products, whereas obese dogs have a different gut microbiota compared to lean dogs. Still, the impact of high dietary protein on gut microbiota in obese dogs remains unclear. The aim of this study was to investigate faecal microbial changes in lean and obese dogs in response to two different levels of dietary protein.Six healthy lean and six obese Beagles were fed a high protein diet (HP) and a low protein diet (LP) for 28 days each in a crossover design. Denaturing gradient gel electrophoresis and quantitative PCR were performed on faecal samples for microbial profiling. Plasma acylcarnitine and fermentation metabolites were measured.ResultsDogs fed HP had higher concentrations of protein fermentation metabolites including faecal ammonia, isovalerate, isobutyrate, phenol, indole, serum indoxyl sulphate and plasma 3-OH isovalerylcarnitine compared to dogs fed LP, whereas no changes in faecal concentrations of acetate and butyrate were observed. The abundances of clostridial clusters IV and XIVa, covering the majority of butyrate-producing bacteria, and of the butyrate kinase gene, one of the terminal genes of the butyrate synthesis pathway were higher in dogs on HP compared to LP. Significant interactions between diet and body condition were found for the abundance of Firmicutes, Lactobacillus and clostridial cluster I. The similarity coefficient of faecal microbiota between the two diets was smaller in obese dogs than in lean dogs.ConclusionsHigh protein diet increased the abundance and activity of butyrate-producing bacteria in Beagles independent of the body condition. In addition, increasing dietary protein content had a greater overall impact on faecal microbiota in obese compared to lean dogs.

【 授权许可】

CC BY   
© The Author(s). 2017