Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection

Malaria Journal

Research

Rintis Noviyanti¹  Andreas Kusuma¹  Jutta Marfurt²  Gabriela Minigo²  Nicholas M. Anstey²  Irene Handayuni²  Zuleima Pava²  Steven Kho²  Kim A. Piera²  Tonia Woodberry²  Ric N. Price³  Christian R. Engwerda⁴  Daniel A. Lampah⁵  Faustina H. Burdam⁵  Enny Kenangalem⁶  Jeanne R. Poespoprodjo⁷ 

[1] Eijkman Institute for Molecular Biology, Jakarta, Indonesia;Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia;Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia;Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, UK;QIMR Berghofer Medical Research Institute, Brisbane, Australia;Timika Malaria Research Programme, Papuan Health and Community Development Foundation, Timika, Papua, Indonesia;Timika Malaria Research Programme, Papuan Health and Community Development Foundation, Timika, Papua, Indonesia;Rumah Sakit Umum Daerah Kabupaten Mimika, Timika, Papua, Indonesia;Timika Malaria Research Programme, Papuan Health and Community Development Foundation, Timika, Papua, Indonesia;Rumah Sakit Umum Daerah Kabupaten Mimika, Timika, Papua, Indonesia;Department of Paediatrics, University of Gadjah Mada, Yogyakarta, Indonesia;

关键词: Malaria;    Clinical immunity;    Dendritic cell;    Regulatory T cell;    Asymptomatic;    Sub-microscopic;    Adults;    Vivax;    Falciparum;    Human;   

DOI  :  10.1186/s12936-016-1382-7

 received in 2016-03-09, accepted in 2016-06-10,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPlasmodium falciparum and Plasmodium vivax infections compromise dendritic cell (DC) function and expand regulatory T (Treg) cells in both clinical disease (malaria) and experimental human sub-microscopic infection. Conversely, in asymptomatic microscopy-positive (patent) P. falciparum or P. vivax infection in endemic areas, blood DC increase or retain HLA-DR expression and Treg cells exhibit reduced activation, suggesting that DC and Treg cells contribute to the control of patent asymptomatic infection. The effect of sub-microscopic (sub-patent) asymptomatic Plasmodium infection on DC and Treg cells in malaria-endemic area residents remains unclear.MethodsIn a cross-sectional household survey conducted in Papua, Indonesia, 162 asymptomatic adults were prospectively evaluated for DC and Treg cells using field-based flow cytometry. Of these, 161 individuals (99 %) were assessed retrospectively by polymerase chain reaction (PCR), 19 of whom had sub-microscopic infection with P. falciparum and 15 with sub-microscopic P. vivax infection. Flow cytometric data were re-analysed after re-grouping asymptomatic individuals according to PCR results into negative controls, sub-microscopic and microscopic parasitaemia to examine DC and Treg cell phenotype in sub-microscopic infection.ResultsAsymptomatic adults with sub-microscopic P. falciparum or P. vivax infection had DC HLA-DR expression and Treg cell activation comparable to PCR-negative controls. Sub-microscopic P. falciparum infection was associated with lower peripheral CD4+ T cells and lymphocytes, however sub-microscopic Plasmodium infection had no apparent effect on DC sub-set number or Treg cell frequency.ConclusionsIn contrast to the impairment of DC maturation/function and the activation of Treg cells seen with sub-microscopic parasitaemia in primary experimental human Plasmodium infection, no phenotypic evidence of dysregulation of DC and Treg cells was observed in asymptomatic sub-microscopic Plasmodium infection in Indonesian adults. This is consistent with DC and Treg cells retaining their functional capacity in sub-microscopic asymptomatic infection with P. falciparum or P. vivax in malaria-endemic areas.

【 授权许可】

CC BY   
© The Author(s) 2016