Effects of X-ray on the metacestodes of Echinococcus granulosus in vitro

BMC Infectious Diseases

Research Article

Mingyang Shu¹  Yongxing Bao¹  Yiwei Bai¹  Rui Mao¹  Aimudula Ainiwaer¹  Ge Wu¹  Pengfei Lu¹  Haitao Li²  Jun Li²  Hui Wang²  Wenbao Zhang² 

[1] Department of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, 830054, Urumqi, China;State Key Laboratory Incubation Base for Xinjiang Major Diseases Research and Xinjiang Key Laboratory of Echinococcosis, The First Affiliated Hospital of Xinjiang Medical University, 830054, Urumqi, China;

关键词: X-ray;    Echinococcus granulosus;    Metacestode;    Infection;   

DOI  :  10.1186/s12879-017-2741-x

 received in 2016-10-31, accepted in 2017-09-15,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundRadiotherapy may represent an alternative treatment modality for cystic echinococcosis (CE), but there is no adequate evidence for it up to now. In this study, we aim to investigate the parasiticidal effects of X-ray on the metacestodes of Echinococcus granulosus in vitro.MethodsProtoscoleces obtained from sheep naturally infected with CE were cultivated in RPMI 1640 medium containing 10% fetal bovine serum (FBS) at 37 °C in 5% CO2. Upon encystation on day 14, the metacestodes were subjected to various intensities of X-ray. Metacestode structures were observed using light microscope and transmission electron microscopy (TEM), and Real-Time PCR was carried out to determine the expression of EgTPX, EgHSP70, EgEPC1 and Caspase-3.ResultsOn day 14, encystation was noticed in the majority of protoscoleces in the control group. In the X-ray groups, the encystation rate showed significant decrease compared with that of the control group (P < 0.05), especially the groups subjected to a dose of ≥40 Gy (P < 0.01). Light microscope findings indicated the hooklets on the rostellum were deranged in the irradiation group, and malformation was noticed in the suckers in a dose dependent manner. For the TEM findings, the cellular structure of the germinal layer of the cysts was completely interrupted by X-ray on day 7. The expression of EgTPX, EgHSP70, EgEPC1 and Caspase-3 was up-regulated after irradiation, especially at a dose of ≥45Gy (P < 0.05).ConclusionsX-ray showed parasiticidal effects on the metacestodes of E. granulosus. Irradiation triggered increased expression of EgTPX, EgHSP70, EgEPC1 and Caspase-3.

【 授权许可】

CC BY   
© The Author(s). 2017