Neuronal and astrocytic interactions modulate brain endothelial properties during metabolic stresses of in vitro cerebral ischemia

Cell Communication and Signaling

Research

Jonathan S Alexander¹  Ganta Vijay Chaitanya²  Alireza Minagar³ 

[1] Department of Molecular and Cellular Physiology, Louisiana State University Health-Shreveport, 71103, Louisiana, USA;Department of Molecular and Cellular Physiology, Louisiana State University Health-Shreveport, 71103, Louisiana, USA;Robert M. Berne Cardiovascular Research Center, Department of Biomedical Engineering, University of Virginia, 22908, Charlottesville, VA, USA;Department of Neurology, Louisiana State University-Health, 71130, Shreveport, LA, USA;

关键词: Cerebral Ischemia;    Blood Brain Barrier Permeability;    Brain Endothelial Cell;    Roswell Park Memorial Institute;    Neurovascular Unit;   

DOI  :  10.1186/1478-811X-12-7

 received in 2013-07-17, accepted in 2013-11-25,  发布年份 2014

来源: Springer

【 摘 要 】

Neurovascular and gliovascular interactions significantly affect endothelial phenotype. Physiologically, brain endothelium attains several of its properties by its intimate association with neurons and astrocytes. However, during cerebrovascular pathologies such as cerebral ischemia, the uncoupling of neurovascular and gliovascular units can result in several phenotypical changes in brain endothelium. The role of neurovascular and gliovascular uncoupling in modulating brain endothelial properties during cerebral ischemia is not clear. Specifically, the roles of metabolic stresses involved in cerebral ischemia, including aglycemia, hypoxia and combined aglycemia and hypoxia (oxygen glucose deprivation and re-oxygenation, OGDR) in modulating neurovascular and gliovascular interactions are not known. The complex intimate interactions in neurovascular and gliovascular units are highly difficult to recapitulate in vitro. However, in the present study, we used a 3D co-culture model of brain endothelium with neurons and astrocytes in vitro reflecting an intimate neurovascular and gliovascular interactions in vivo. While the cellular signaling interactions in neurovascular and gliovascular units in vivo are much more complex than the 3D co-culture models in vitro, we were still able to observe several important phenotypical changes in brain endothelial properties by metabolically stressed neurons and astrocytes including changes in barrier, lymphocyte adhesive properties, endothelial cell adhesion molecule expression and in vitro angiogenic potential.

【 授权许可】

Unknown   
© Chaitanya et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.