Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation

Reproductive Biology and Endocrinology

Research

Federica Moffa¹  Julie Dechène²  Yvon Englert³  Isabelle Demeestere³  Giovanna Fasano³ 

[1] Fertility Clinic, Department of Obstetrics and Gynaecology, Erasme Hospital Université Libre de Bruxelles, 1070, Brussels, Belgium;Research Laboratory on Human Reproduction, Faculty of Medicine, Campus Erasme, Université Libre de Bruxelles, 1070, Brussels, Belgium;Research Laboratory on Human Reproduction, Faculty of Medicine, Campus Erasme, Université Libre de Bruxelles, 1070, Brussels, Belgium;Fertility Clinic, Department of Obstetrics and Gynaecology, Erasme Hospital Université Libre de Bruxelles, 1070, Brussels, Belgium;

关键词: Ovarian Tissue;    Follicular Fluid;    Antral Follicle;    Primordial Follicle;    Germinal Vesicle;   

DOI  :  10.1186/1477-7827-9-150

 received in 2011-09-05, accepted in 2011-11-23,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundIn the past few years, cryopreservation of ovarian tissue has become an established procedure proposed in many centers around the world and transplantation has successfully resulted in full-term pregnancies and deliveries in human. This prospective study aims to evaluate the feasibility of vitrifying in vitro matured oocytes (IVM) isolated at the time of ovarian tissue cryopreservation to improve the efficiency of fertility preservation programs.MethodsOocyte-cumulus complexes were retrieved from freshly collected ovarian cortex by aspirating antral follicular fluid, and were matured in vitro for 24-48 h prior to vitrification. Oocytes were matured in an IVM commercial medium (Copper Surgical, USA) supplemented with 75 mIU/ml FSH and 75 mIU/ml LH and vitrified using a commercial vitrification kit (Irvine Scientific, California) in high security vitrification straws (CryoBioSystem, France). Oocyte collection and IVM rates were evaluated according to the age, the cycle period and the amount of tissue collected.ResultsImmature oocyte retrieval from ovarian tissue was carried out in 57 patients between 8 and 35 years of age, undergoing ovarian tissue cryopreservation. A total of 266 oocytes were isolated, 28 of them were degenerated, 200 were at germinal vesicle stage (GV), 35 were in metaphase I (MI) and 3 displayed a visible polar body (MII). The number of oocytes collected was positively correlated with the amount of tissue cryopreserved (p < 0.001) and negatively correlated with the age of the patients (p = 0.005). Oocytes were obtained regardless of menstrual cycle period or contraception. A total maturation rate of 31% was achieved, leading to the vitrification of at least one mature oocyte for half of the cohort.ConclusionsThe study showed that a significant number of immature oocytes can be collected from excised ovarian tissue whatever the menstrual cycle phases and the age of the patients, even for prepubertal girls.

【 授权许可】

CC BY   
© Fasano et al; licensee BioMed Central Ltd. 2011