Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins

Proteome Science

Research

Chao Qiu¹  Xiaojun Zhang²  Jianjun Sun³  Hongzhou Lu³  Xia Peng³  Lin Yin³  Yamin Yao³  Xiaofang Jia³  Tangkai Qi³  Fang Ma³  Lijun Zhang³ 

[1] Institutes of Biomedical Sciences, Fudan University, 200032, Shanghai, PR China;Neurosurgery, Fuzhou General Hospital, 350025, Fuzhou, PR China;Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China;

关键词: Healthy Donor;    Protein Spot;    Host Cell Protein;    Ficoll Density Gradient Centrifugation;    Host Cell Apoptosis;   

DOI  :  10.1186/1477-5956-8-12

 received in 2009-11-24, accepted in 2010-03-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls.Results26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.ConclusionsTogether, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.

【 授权许可】

CC BY   
© Zhang et al; licensee BioMed Central Ltd. 2010