A high performance liquid chromatographic assay of Mefloquine in saliva after a single oral dose in healthy adult Africans

Malaria Journal

Methodology

Abayomi Sijuade¹  Grace O Gbotosho¹  Akin Sowunmi¹  Omowunmi Lawal²  Christian T Happi²  Ayoade Oduola³ 

[1] Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria;Malaria Research Laboratories, Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria;Malaria Research Laboratories, Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria;Stewardship for Research, Tropical Disease Research (TDR), World Health Organization, Geneva, Switzerland;

关键词: Malaria;    Mefloquine;    Saliva;    High performance liquid chromatography;   

DOI  :  10.1186/1475-2875-11-59

 received in 2011-10-17, accepted in 2012-02-27,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMefloquine-artesunate is a formulation of artemisinin based combination therapy (ACT) recommended by the World Health Organization and historically the first ACT used clinically. The use of ACT demands constant monitoring of therapeutic efficacies and drug levels, in order to ensure that optimum drug exposure is achieved and detect reduced susceptibility to these drugs. Quantification of anti-malarial drugs in biological fluids other than blood would provide a more readily applicable method of therapeutic drug monitoring in developing endemic countries. Efforts in this study were devoted to the development of a simple, field applicable, non-invasive method for assay of mefloquine in saliva.MethodsA high performance liquid chromatographic method with UV detection at 220 nm for assaying mefloquine in saliva was developed and validated by comparing mefloquine concentrations in saliva and plasma samples from four healthy volunteers who received single oral dose of mefloquine. Verapamil was used as internal standard. Chromatographic separation was achieved using a Hypersil ODS column.ResultsExtraction recoveries of mefloquine in plasma or saliva were 76-86% or 83-93% respectively. Limit of quantification of mefloquine was 20 ng/ml. Agreement between salivary and plasma mefloquine concentrations was satisfactory (r = 0.88, p < 0.001). Saliva:plasma concentrations ratio was 0.42.ConclusionDisposition of mefloquine in saliva paralleled that in plasma, making salivary quantification of mefloquine potentially useful in therapeutic drug monitoring.

【 授权许可】

CC BY   
© Gbotosho et al; licensee BioMed Central Ltd. 2012