Yeast glucose pathways converge on the transcriptional regulation of trehalose biosynthesis

BMC Genomics

Research Article

Patrick Kemmeren¹  Katrin Sameith¹  Nathalie Brabers¹  Eva Apweiler¹  Dik van Leenen¹  Frank CP Holstege¹  Loes van de Pasch¹  Thanasis Margaritis¹  Linda V Bakker² 

[1] Molecular Cancer Research, University Medical Centre Utrecht, Universiteitsweg 100, Utrecht, the Netherlands;Molecular Cancer Research, University Medical Centre Utrecht, Universiteitsweg 100, Utrecht, the Netherlands;Netherlands Bioinformatics Centre, Geert Grooteplein 28, 6525, Nijmegen, GA, the Netherlands;

关键词: Regulatory networks;    Glucose signalling;    Trehalose biosynthesis;    Gene expression profiling;    Saccharomyces cerevisiae;   

DOI  :  10.1186/1471-2164-13-239

 received in 2012-01-03, accepted in 2012-06-14,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCellular glucose availability is crucial for the functioning of most biological processes. Our understanding of the glucose regulatory system has been greatly advanced by studying the model organism Saccharomyces cerevisiae, but many aspects of this system remain elusive. To understand the organisation of the glucose regulatory system, we analysed 91 deletion mutants of the different glucose signalling and metabolic pathways in Saccharomyces cerevisiae using DNA microarrays.ResultsIn general, the mutations do not induce pathway-specific transcriptional responses. Instead, one main transcriptional response is discerned, which varies in direction to mimic either a high or a low glucose response. Detailed analysis uncovers established and new relationships within and between individual pathways and their members. In contrast to signalling components, metabolic components of the glucose regulatory system are transcriptionally more frequently affected. A new network approach is applied that exposes the hierarchical organisation of the glucose regulatory system.ConclusionsThe tight interconnection between the different pathways of the glucose regulatory system is reflected by the main transcriptional response observed. Tps2 and Tsl1, two enzymes involved in the biosynthesis of the storage carbohydrate trehalose, are predicted to be the most downstream transcriptional components. Epistasis analysis of tps2 Δ double mutants supports this prediction. Although based on transcriptional changes only, these results suggest that all changes in perceived glucose levels ultimately lead to a shift in trehalose biosynthesis.

【 授权许可】

Unknown   
© Apweiler et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.