MicroDSC study of Staphylococcus epidermidis growth

BMC Microbiology

Research Article

Vlad T Popa¹  Octavian Balint²  Cezar Iancu²  Miron A Bogdan²  Dragos C Zaharia²  Alexandru A Muntean²  Alexandru T Steriade²  Mircea I Popa² 

[1] Romanian Academy, Institute of Physical Chemistry „Ilie Murgulescu", Splaiul Independentei 202, 060021, Bucharest, Romania;University of Medicine and Pharmacy „Carol Davila", Eroii Sanitari Bulevard 8, Bucharest, Romania;

关键词: Cold Storage;    Bacterial Concentration;    Thermal Signal;    Signal Perturbation;    Ramp Heating;   

DOI  :  10.1186/1471-2180-10-322

 received in 2010-05-18, accepted in 2010-12-17,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundA microcalorimetric study was carried out using a Staphylococcus epidermidis population to determine the reproducibility of bacterial growth and the variability of the results within certain experimental parameters (temperature, bacterial concentration, sample thermal history). Reproducibility tests were performed as series of experiments within the same conditions using either freshly prepared populations or samples kept in cold storage. In both cases, the samples were obtained by serial dilution from a concentrated TSB bacterial inoculum incubated overnight.ResultsThe results show that experiments are fairly reproducible and that specimens can be preserved at low temperatures (1 - 2°C) at least 4 days. The thermal signal variations at different temperatures and initial bacterial concentrations obey a set of rules that we identified.ConclusionOur study adds to the accumulating data and confirms available results of isothermal microcalorimetry applications in microbiology and can be used to standardize this method for either research or clinical setting.

【 授权许可】

Unknown   
© Zaharia et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.