AtROP1 negatively regulates potato resistance to Phytophthora infestans via NADPH oxidase-mediated accumulation of H2O2

BMC Plant Biology

Research Article

Jing Gao¹  Mingshou Fan¹  Fan Yang¹  Ren Na¹  Xiaoluo Zhang¹  Jun Zhao¹  Zhiwei Zhang¹  Shuqing Yang¹  Yan Zhao² 

[1] Department of Agronomy, Inner Mongolia Agricultural University, 010019, Huhhot, Inner Mongolia, China;Institutes of Genetics and Developmental Biology, Chinese Academy of Science, 100101, Beijing, China;

关键词: AtRop1;    Potato;    Resistance;    NADPH oxidase;    H2O2 production;    Jasmonic acid;   

DOI  :  10.1186/s12870-014-0392-2

 received in 2014-08-25, accepted in 2014-12-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundSmall GTPases are monomeric guanine nucleotide-binding proteins. In plants, ROPs regulate plant cell polarity, plant cell differentiation and development as well as biotic and abiotic stress signaling pathways.ResultsWe report the subcellular localization of the AtRop1 protein at the plasma membrane in tobacco epidermal cells using GFP fusions. Additionally, transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1 in potato led to H2O2 accumulation associated with the reduced development of Phytophthora infestans Montagne de Bary and smaller lesions on infected potato leaves. The expression of the Strboh-D gene, a NADPH oxidase homologue in potato, was analyzed by RT-PCR. Expression of this gene was maintained in DN-AtRop1 transgenic plants after infection with P. infestans. In transgenic potato lines, the transcript levels of salicylic acid (SA) and jasmonic acid (JA) marker genes (Npr1 and Lox, respectively) were analyzed. The Lox gene was induced dramatically whereas expression of Npr1, a gene up-regulated by SA, decreased slightly in DN-AtRop1 transgenic plants after infection with P. infestans.ConclusionsIn conclusion, our results indicate that DN-AtROP1 affects potato resistance to P. infestans. This is associated with increased NADPH oxidase-mediated H2O2 production and JA signaling.

【 授权许可】

Unknown   
© Zhang et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.