Activation of conventional protein kinase C (PKC) is critical in the generation of human neutrophil extracellular traps

Journal of Inflammation

Short Report

Feng Li¹  Robert D Gray¹  Chris Haslett¹  Christopher D Lucas¹  Annie MacKellar¹  Donald J Davidson¹  Katia Hiersemenzel¹  Adriano G Rossi¹ 

[1] MRC Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh Medical School, 47 Little France Crescent, Edinburgh, Scotland, UK;

关键词: NADPH Oxidase;    Chronic Granulomatous Disease;    Neutrophil Extracellular Trap;    Neutrophil Extracellular Trap Formation;    SYTOX Green;   

DOI  :  10.1186/1476-9255-10-12

 received in 2012-09-04, accepted in 2013-02-22,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundActivation of NADPH oxidase is required for neutrophil extracellular trap (NET) formation. Protein kinase C (PKC) is an upstream mediator of NADPH oxidase activation and thus likely to have a role in NET formation.MethodsPharmacological inhibitors were used to block PKC activity in neutrophils harvested from healthy donor blood.ResultsPan PKC inhibition with Ro-31-8220 (p<0.001), conventional PKC inhibition with Go 6976 (p<0.001) and specific PKCβ inhibition with LY333531 (p<0.01) blocked NET formation in response to PMA. Inhibition of novel and atypical PKC had no effect. LY333531 blocked NET induction by the diacylglycerol analogue OAG (conventional PKC activator) (p<0.001).ConclusionsConventional PKCs have a prominent role in NET formation. Furthermore PKCβ is the major isoform implicated in NET formation.

【 授权许可】

CC BY   
© Gray et al.; licensee BioMed Central Ltd. 2013