Journal of Inflammation | |
Activation of conventional protein kinase C (PKC) is critical in the generation of human neutrophil extracellular traps | |
Short Report | |
Feng Li1  Robert D Gray1  Chris Haslett1  Christopher D Lucas1  Annie MacKellar1  Donald J Davidson1  Katia Hiersemenzel1  Adriano G Rossi1  | |
[1] MRC Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh Medical School, 47 Little France Crescent, Edinburgh, Scotland, UK; | |
关键词: NADPH Oxidase; Chronic Granulomatous Disease; Neutrophil Extracellular Trap; Neutrophil Extracellular Trap Formation; SYTOX Green; | |
DOI : 10.1186/1476-9255-10-12 | |
received in 2012-09-04, accepted in 2013-02-22, 发布年份 2013 | |
来源: Springer | |
【 摘 要 】
BackgroundActivation of NADPH oxidase is required for neutrophil extracellular trap (NET) formation. Protein kinase C (PKC) is an upstream mediator of NADPH oxidase activation and thus likely to have a role in NET formation.MethodsPharmacological inhibitors were used to block PKC activity in neutrophils harvested from healthy donor blood.ResultsPan PKC inhibition with Ro-31-8220 (p<0.001), conventional PKC inhibition with Go 6976 (p<0.001) and specific PKCβ inhibition with LY333531 (p<0.01) blocked NET formation in response to PMA. Inhibition of novel and atypical PKC had no effect. LY333531 blocked NET induction by the diacylglycerol analogue OAG (conventional PKC activator) (p<0.001).ConclusionsConventional PKCs have a prominent role in NET formation. Furthermore PKCβ is the major isoform implicated in NET formation.
【 授权许可】
CC BY
© Gray et al.; licensee BioMed Central Ltd. 2013
【 预 览 】
Files | Size | Format | View |
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RO202311106282829ZK.pdf | 1878KB | download |
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