Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

Journal of Nanobiotechnology

Research

Huan-Cheng Chang¹  Giang Thu Nguyen²  Thuong Thi Ho²  Ngoc Thu Le²  Thuy Thi Le²  Ngoc Bich Pham³  Ha Hoang Chu³  Minh Dinh Pham⁴  Udo Conrad⁵ 

[1] Institute of Atomic and Molecular Sciences, Academia Sinica, 10617, Taipei, Taiwan, ROC;Institute of Biotechnology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam;Institute of Biotechnology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam;Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam;Institute of Biotechnology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam;Institute of Atomic and Molecular Sciences, Academia Sinica, 10617, Taipei, Taiwan, ROC;Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany;

关键词: Nanodiamond;    Recombinant H7 haemagglutinin;    Influenza virus A/H7N9;    Synthetic virus-like particles;    Plant-derived protein;   

DOI  :  10.1186/s12951-017-0305-2

 received in 2017-04-10, accepted in 2017-09-23,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe continuing spread of the newly emerged H7N9 virus among poultry in China, as well as the possibility of human-to-human transmission, has attracted numerous efforts to develop an effective vaccine against H7N9. The use of nanoparticles in vaccinology is inspired by the fact that most pathogens have a dimension within the nano-size range and therefore can be processed efficiently by the immune system, which leads to a potent immune response. Herein, we report a facile approach to increase antigen size to achieve not only fast but also effective responses against the recombinant HA/H7N9 protein via a simple conjugation of the protein onto the surface of nanodiamond particles.ResultsIn this study, trimeric Haemagglutinin (H7) that is transiently expressed in N. benthamiana was purified using affinity chromatography, and its trimeric state was revealed successfully by the cross-linking reaction. The trimeric H7 solution was subsequently mixed with a nanodiamond suspension in different ratios. The successful conjugation of the trimeric H7 onto the surface of nanodiamond particles was demonstrated by the changes in size and Zeta-potential of the particles before and after protein coating, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Western-blot analysis. Next, biofunction of the protein-nanodiamond conjugates was screened using a haemagglutination assay. A mixture containing 5 µg of trimeric H7 and 60 µg of nanodiamond corresponds to a ratio of 1:12 (w/w) of agglutinated chicken red blood cells at HA titer of 1024, which is 512-fold higher than the HA titer of free trimeric H7. After the 2nd and 3rd immunization in mice, ELISA and Western blot analyses demonstrated that the physical mixture of trimeric H7 protein and nanodiamond (1:12, w/w) elicited statistically significant stronger H7-specific-IgG response demonstrated by higher amounts of H7N9-specific IgG (over 15.4-fold with P < 0.05 after the second immunization).ConclusionsThese results indicated a potential effect inherent to nanodiamond towards modulating immune systems, which should be further evaluated and broadly applied in nanovaccine development.

【 授权许可】

CC BY   
© The Author(s) 2017