Expression of fourteen novel obesity-related genes in zucker diabetic fatty rats

Cardiovascular Diabetology

Original Investigation

Iris Heid¹  Christa Buechler²  Dierk H Endemann³  Markus Resch³  Andreas Luchner³  Guenter A Riegger³  Andreas Steege³  Christoph Birner³  Peter M Schmid⁴ 

[1] Institut für Epidemiologie und Präventivmedizin, University of Regensburg, Regensburg, Germany;Klinik und Poliklinik für Innere Medizin I, University of Regensburg, Regensburg, Germany;Klinik und Poliklinik für Innere Medizin II, University of Regensburg, Regensburg, Germany;Klinik und Poliklinik für Innere Medizin II, University of Regensburg, Regensburg, Germany;Klinik und Poliklinik für Innere Medizin II, Franz-Josef-Strauss-Allee 11, University of Regensburg, 93042, Regensburg, Germany;

关键词: BDNF;    ETV5;    FAIM2;    FTO;    GNPDA2;    KCTD15;    LYPLAL1;    MCR4;    MTCH2;    NEGR1;    NRXN3;    TMEM18;    SEC16B;    TFAP2B;    ZDF-rats;   

DOI  :  10.1186/1475-2840-11-48

 received in 2012-01-28, accepted in 2012-05-03,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundGenome-wide association studies (GWAS) are useful to reveal an association between single nucleotide polymorphisms and different measures of obesity. A multitude of new loci has recently been reported, but the exact function of most of the according genes is not known. The aim of our study was to start elucidating the function of some of these genes.MethodsWe performed an expression analysis of fourteen genes, namely BDNF, ETV5, FAIM2, FTO, GNPDA2, KCTD15, LYPLAL1, MCR4, MTCH2, NEGR1, NRXN3, TMEM18, SEC16B and TFAP2B, via real-time RT-PCR in adipose tissue of the kidney capsule, the mesenterium and subcutaneum as well as the hypothalamus of obese Zucker diabetic fatty (ZDF) and Zucker lean (ZL) rats at an age of 22 weeks.ResultsAll of our target genes except for SEC16B showed the highest expression in the hypothalamus. This suggests a critical role of these obesity-related genes in the central regulation of energy balance. Interestingly, the expression pattern in the hypothalamus showed no differences between obese ZDF and lean ZL rats. However, LYPLAL1, TFAP2B, SEC16B and FAIM2 were significantly lower expressed in the kidney fat of ZDF than ZL rats. NEGR1 was even lower expressed in subcutaneous and mesenterial fat, while MTCH2 was higher expressed in the subcutaneous and mesenterial fat of ZDF rats.ConclusionThe expression pattern of the investigated obesity genes implies for most of them a role in the central regulation of energy balance, but for some also a role in the adipose tissue itself. For the development of the ZDF phenotype peripheral rather than central mechanisms of the investigated genes seem to be relevant.

【 授权许可】

CC BY   
© Schmid et al.; licensee BioMed Central Ltd. 2012