Multichromosomal mitochondrial genome of Punica granatum: comparative evolutionary analysis and gene transformation from chloroplast genomes

BMC Plant Biology

Research

Chuanzeng Wang¹  Lijuan Feng²  Yanlei Yin²  Zenghui Wang²  Xuemei Yang²  Mengmeng An³ 

[1] Shandong Academy of Agricultural Sciences, 250100, Jinan, Shandong, China;Shandong Institute of Pomology, 271000, Taian, Shandong, China;Zibo Academy of Agricultural Sciences, 255000, Zibo, Shandong, China;

关键词: Punica granatum;    Mitogenome;    Multi-chromosomal structure;    Evolutionary relationship;    Intracellular gene transfer;   

DOI  :  10.1186/s12870-023-04538-8

 received in 2023-07-05, accepted in 2023-10-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundPunica granatum is a fundamentally important fruit tree that has important economic, medicinal and ornamental properties. At present, there are few reports on the mitochondrial genome of pomegranate. Hence, in this study the P. granatum mitogenome was sequenced and assembled to further understanding of organization, variation, and evolution of mitogenomes of this tree species.ResultsThe genome structure was multi-chromosomes with seven circular contigs, measuring 382,774 bp in length with a 45.91% GC content. It contained 74 genes, including 46 protein-coding genes, 25 tRNA genes, and three rRNA genes. There were 188 pairs of dispersed repeats with lengths of 30 or greater, primarily consisting of reverse complementary repeats. The mitogenome analysis identified 114SSRs and 466 RNA editing sites. Analyses of codon usage, nucleotide diversity and gene migration from chloroplast to mitochondrial were also conducted. The collinear and comparative analysis of mitochondrial structures between P. granatum and its proximal species indicated that P. granatum ‘Taishanhong’ was closely related to P. granatum ‘Qingpitian’ and Lagerstroemia indica. Phylogenetic examination based on the mitogenome also confirmed the evolutionary relationship.ConclusionThe results offered crucial information on the evolutionary biology of pomegranate and highlighted ways to promote the utilization of the species’ germplasm.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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