Genomic survey, characterization and expression profile analysis of the peptide transporter family in rice (Oryza sativa L.)

BMC Plant Biology

Research Article

Huihui Yu¹  Lei Wang¹  Weibo Xie¹  Jianyan Huang¹  Xiaobo Zhao¹ 

[1] National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China;

关键词: Tandem Duplication;    Segmental Duplication;    Peptide Transporter;    Nitrate Transporter;    Phytohormone Treatment;   

DOI  :  10.1186/1471-2229-10-92

 received in 2010-01-22, accepted in 2010-05-20,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPeptide transporter (PTR) family whose member can transport di-/tripeptides and nitrate is important for plant growth and development. Although the rice (Oryza sativa L.) genome has been sequenced for a few years, a genomic survey, characterization and expression profile analysis of the PTR family in this species has not been reported.ResultsIn this study, we report a comprehensive identification, characterization, phylogenetic and evolutionary analysis of 84 PTR family members in rice (OsPTR) as well as their whole-life expression patterns. Chromosomal distribution and sequence analysis indicate that nearly 70% of OsPTR members are involved in the tandem and segmental duplication events. It suggests that genome duplication might be a major mechanism for expansion of this family. Highly conserved motifs were identified in most of the OsPTR members. Meanwhile, expression profile of OsPTR genes has been analyzed by using Affymetrix rice microarray and real-time PCR in two elite hybrid rice parents, Minghui 63 and Zhenshan 97. Seven genes are found to exhibit either preferential or tissue-specific expression during different development stages of rice. Under phytohormone (NAA, GA3 and KT) and light/dark treatments, 14 and 17 OsPTR genes are differentially expressed respectively. Ka/Ks analysis of the paralogous OsPTR genes indicates that purifying selection plays an important role in function maintenance of this family.ConclusionThese investigations add to our understanding of the importance of OsPTR family members and provide useful reference for selecting candidate genes for functional validation studies of this family in rice.

【 授权许可】

CC BY   
© Zhao et al; licensee BioMed Central Ltd. 2010