Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria

Annals of Clinical Microbiology and Antimicrobials

Research

Kayode Fashae¹  Haluk Vahaboglu²  Fatma Budak³  Fetiye Kolayli³  Sinem Torol⁴  Murat Kasap⁴ 

[1] Department of Botany, Microbiology University of Ibadan, Nigeria;Enfeksiyon Hastaliklari ve Klinik Mikrobiyoloji AD,KOU Tip Fakultesi, Izmit, Turkey;Mikrobiyoloji ve Klinik Mikrobiyoloji AD,KOU Tip Fakultesi, Izmit, Turkey;Tibbi Biyoloji AD,KOU Tip Fakultesi, Izmit, Turkey;

关键词: Cefepime;    Enterobacter Aerogenes;    Clavulanate;    Disk Diffusion Test;    AmpC Enzyme;   

DOI  :  10.1186/1476-0711-9-1

 received in 2009-11-14, accepted in 2010-01-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundWe studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.MethodsMicrobiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.ResultsBy transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.ConclusionThis is the first study demonstrated the occurrence of SHV-12 in Nigeria.

【 授权许可】

CC BY   
© Kasap et al; licensee BioMed Central Ltd. 2010