The signals of FGFs on the neurogenesis of embryonic stem cells

Journal of Biomedical Science

Research

Kun-Hsiung Lee¹  Shinn-Zong Lin²  Ching-Wen Chen³  Shih-Cheng Shen³  Hong-Lin Su⁴  Chin-San Liu⁵  Hung-Chuan Pan⁶  Ing-Ming Chiu⁷ 

[1] Animal Technology Institute Taiwan; Miaoli, Taiwan;Center for Neuropsychiatry, Taiwan; Department of Immunology, China Medical University and Hospital, Taichung, Taiwan; China Medical University Beigang Hospital, Yunlin, China Medical University, Taichung, Taiwan;Department of Life Sciences, National Chung-Hsing University, Taichung, Taiwan;Department of Life Sciences, National Chung-Hsing University, Taichung, Taiwan;Department of Physical Therapy, China Medical University, Taichung, Taiwan;Department of Medical Research, Changhua Christian Hospital, Changhua, Taiwan;Department of Neurosurgery, Taichung Veterans General Hospital; Taichung, Taiwan;Institute of Cellular and Systems Medicine, National Health Research Institutes; Miaoli, Taiwan;

关键词: Embryonic Stem;    Embryonic Stem Cell;    Neural Induction;    Neural Fate;    Neuroblast Cell;   

DOI  :  10.1186/1423-0127-17-33

 received in 2009-12-28, accepted in 2010-04-29,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundNeural induction is a complex process and the detailed mechanism of FGF-induced neurogenesis remains unclear.MethodsBy using a serum-free neural induction method, we showed that FGF1 dose-dependently promoted the induction of Sox1/N-cadherin/nestin triple positive cells, which represent primitive neuroblasts, from mouse embryonic stem (ES) cells.ResultsWe demonstrated that FGF1, FGF2, and FGF4, but not FGF8b, enhanced this neurogenesis. Especially, FGF-enhanced neurogenesis is not mediated through the rescue of the apoptosis or the enhancement of the proliferation of Sox1+ cells. We further indicated that the inactivation of c-Jun N-terminal kinase-1 (JNK-1) and extracellular signal-related kinase-2 (ERK-2), but not p38 mitogen-activated protein kinase (MAPK), inhibited the neural formation through the inhibition of ES differentiation, but not through the formation of endomesodermal cells.ConclusionsThese lines of evidence delineated the roles of FGF downstream signals in the early neural differentiation of ES cells.

【 授权许可】

Unknown   
© Chen et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.