Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence

BMC Plant Biology

Research Article

Zhijian Chang¹  Ling Wang²  Liqin Li²  Zhenglong Ren²  Zongxiang Tang²  Hefei Rao²  Huaiyu Zhang²  Min Zhang²  Shengfu Zhong²  Wanquan Chen³  Taiguo Liu³  Peigao Luo⁴  Xin Li⁴ 

[1] Institute of Crop Genetics, Shanxi Academy of Agricultural Science, 030031, Taiyuan, Shanxi, China;Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, China;Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China;

关键词: Quantitative reverse transcription PCR;    Puccinia striiformis;    Triticum aestivum;    Suppression subtractive hybridization;    Photosynthesis;   

DOI  :  10.1186/s12870-015-0612-4

 received in 2015-05-11, accepted in 2015-09-12,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundStripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.MethodsWe used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.ResultsIn silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.DiscussionSequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.ConclusionsThe unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.

【 授权许可】

CC BY   
© Li et al. 2015