Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus ‘Robusta 5’ accessions

BMC Genetics

Research Article

Yizhen Wan¹  Herb S Aldwinckle²  Mickael Malnoy³  Klaus Richter⁴  Andreas Peil⁵  Mary Horner⁶  Vincent GM Bus⁶  Nihal de Silva⁷  Claudia Wiedow⁸  Charmaine Carlisle⁸  Deepa Bowatte⁸  Susan E Gardiner⁸  Jean-Marc Celton⁹  Carole L Bassett^1,10  John L Norelli^1,10  Gennaro Fazio^1,11  Angela M Baldo^1,11 

[1] Apple Research Center, College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, China;Department of Plant Pathology and Plant-Microbe Biology, Cornell University, 630W. North St., 14456, Geneva, NY, USA;Foundation E. Mach - Istituto Agrario San Michele all'Adige, Via E. Mach 1, 38010, San Michele all'Adige, TN, Italy;JKI, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, D-06484, Quedlinburg, Germany;Julius Kühn-Institut (JKI), Institute for Breeding Research on Horticultural and Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany;PFR Hawke’s Bay, Private Bag 1401, 4157, Havelock North, New Zealand;PFR Mt Albert, Private Bag 92169, Auckland Mail Centre, 1142, Auckland, New Zealand;The New Zealand Institute for Plant & Food Research Limited (PFR) Palmerston North, Private Bag 11600, Manawatu Mail Centre, 4442, Palmerston North, New Zealand;UMR Génétique et Horticulture (GenHort), INRA ⁄ Agrocampus-ouest, Université d’Angers, Centre Angers-Nantes, BP 60057, 42 rue Georges Morel, 49071, Beaucouze´ Cedex, France;USDA-ARS, Appalachian Fruit Research Station, 2217 Wiltshire Rd., 25430, Kearneysville, WV, USA;USDA-ARS, Plant Genetic Resources Unit, 630W. North St., 14456, Geneva, NY, USA;

关键词: Quantitative Trait Locus;    Whole Genome Sequence;    Suppression Subtractive Hybridization;    Composite Interval Mapping;    High Resolution Melting;   

DOI  :  10.1186/1471-2156-13-25

 received in 2011-11-02, accepted in 2012-03-19,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundBreeding of fire blight resistant scions and rootstocks is a goal of several international apple breeding programs, as options are limited for management of this destructive disease caused by the bacterial pathogen Erwinia amylovora. A broad, large-effect quantitative trait locus (QTL) for fire blight resistance has been reported on linkage group 3 of Malus ‘Robusta 5’. In this study we identified markers derived from putative fire blight resistance genes associated with the QTL by integrating further genetic mapping studies with bioinformatics analysis of transcript profiling data and genome sequence databases.ResultsWhen several defined E.amylovora strains were used to inoculate three progenies from international breeding programs, all with ‘Robusta 5’ as a common parent, two distinct QTLs were detected on linkage group 3, where only one had previously been mapped. In the New Zealand ‘Malling 9’ X ‘Robusta 5’ population inoculated with E. amylovora ICMP11176, the proximal QTL co-located with SNP markers derived from a leucine-rich repeat, receptor-like protein ( MxdRLP1) and a closely linked class 3 peroxidase gene. While the QTL detected in the German ‘Idared’ X ‘Robusta 5’ population inoculated with E. amylovora strains Ea222_JKI or ICMP11176 was approximately 6 cM distal to this, directly below a SNP marker derived from a heat shock 90 family protein gene ( HSP90). In the US ‘Otawa3’ X ‘Robusta5’ population inoculated with E. amylovora strains Ea273 or E2002a, the position of the LOD score peak on linkage group 3 was dependent upon the pathogen strains used for inoculation. One of the five MxdRLP1 alleles identified in fire blight resistant and susceptible cultivars was genetically associated with resistance and used to develop a high resolution melting PCR marker. A resistance QTL detected on linkage group 7 of the US population co-located with another HSP90 gene-family member and a WRKY transcription factor previously associated with fire blight resistance. However, this QTL was not observed in the New Zealand or German populations.ConclusionsThe results suggest that the upper region of ‘Robusta 5’ linkage group 3 contains multiple genes contributing to fire blight resistance and that their contributions to resistance can vary depending upon pathogen virulence and other factors. Mapping markers derived from putative fire blight resistance genes has proved a useful aid in defining these QTLs and developing markers for marker-assisted breeding of fire blight resistance.

【 授权许可】

CC BY   
© Gardiner et al.; licensee BioMed Central Ltd. 2012