BMI1 and Mel-18 oppositely regulate carcinogenesis and progression of gastric cancer

Molecular Cancer

Research

Yu-Fan Cheng¹  Xiao-Wei Zhang¹  Wei-Jian Guo¹  You-Wei Lu¹  Jin Li¹  Wei Qin²  Feng-Chun Zhang²  Qian Li³  Ya-Ping Sheng³  Goberdhan P Dimri⁴  Bing-Ya Liu⁵ 

[1] Department of Medical Oncology, Cancer Hospital of Fudan University, 270 Dong An Road, 200032, Shanghai, China;Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China;Department of Medical Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 200001, Shanghai, China;Department of Medical Oncology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, 200092, Shanghai, China;Department of Medicine, NorthShore University HealthSystem Research Institute, 1001 University Place, 60201, Evanston, IL, USA;Shanghai institute of digestive surgery, 200025, Shanghai, China;

关键词: Gastric Cancer;    Gastric Cancer Cell;    Gastric Cancer Cell Line;    Gastric Tumor;    Gastric Cancer Tissue;   

DOI  :  10.1186/1476-4598-9-40

 received in 2009-10-07, accepted in 2010-02-21,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe BMI1 oncogene is overexpressed in several human malignancies including gastric cancer. In addition to BMI1, mammalian cells also express Mel-18, which is closely related to BMI1. We have reported that Mel-18 functions as a potential tumor suppressor by repressing the expression of BMI1 and consequent downregulation of activated AKT in breast cancer cells. However, the mechanisms of BMI1 overexpression and the role of Mel-18 in other cancers are still not clear. The purpose of this study is to investigate the role of BMI1 and Mel-18 in gastric cancer.ResultsBMI1 was found to be overexpressed in gastric cancer cell lines and gastric tumors. Overexpression of BMI1 correlated with advanced clinical stage and lymph node metastasis; while the expression of Mel-18 negatively correlated with BMI1. BMI1 but not Mel-18 was found to be an independent prognostic factor. Downregulation of BMI1 by Mel-18 overexpression or knockdown of BMI1 expression in gastric cancer cell lines led to upregulation of p16 (p16INK4a or CDKN2A) in p16 positive cell lines and reduction of phospho-AKT in both p16-positive and p16-negative cell lines. Downregulation of BMI1 was also accompanied by decreased transformed phenotype and migration in both p16- positive and p16-negative gastric cancer cell lines.ConclusionsIn the context of gastric cancer, BMI1 acts as an oncogene and Mel-18 functions as a tumor suppressor via downregulation of BMI1. Mel-18 and BMI1 may regulate tumorigenesis, cell migration and cancer metastasis via both p16- and AKT-dependent growth regulatory pathways.

【 授权许可】

Unknown   
© Zhang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.