Characterizing cancer cells with cancer stem cell-like features in 293T human embryonic kidney cells

Molecular Cancer

Research

Naoto T Ueno¹  Massimo Cristofanilli¹  Fredika Robertson²  Hui Gao³  Evan Cohen³  James M Reuben³  Savitri Krishnamurthy⁴  Wendy A Woodward⁵  Bisrat G Debeb⁵  Wei Xu⁵  Li Li⁵  Lara Lacerda⁵  Thomas A Buchholz⁵  Anthony Lucci⁶  Angel A Rodriguez⁷  Melissa D Landis⁷  Michael T Lewis⁷  Xiaomei Zhang⁷ 

[1] Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA;Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA;Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA;Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA;Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA;Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA;Lester and Sue Smith Breast Center and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA;

关键词: 293T Cell;    Cancer Stem Cell;    ALDH Activity;    Cancer Stem Cell Marker;    Cancer Stem Cell Population;   

DOI  :  10.1186/1476-4598-9-180

 received in 2010-04-29, accepted in 2010-07-08,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSince the first suggestion of prospectively identifiable cancer stem cells in solid tumors, efforts have been made to characterize reported cancer stem cell surrogates in existing cancer cell lines, and cell lines rich with these surrogates have been used to screen for cancer stem cell targeted agents. Although 293T cells were derived from human embryonic kidney, transplantation of these cells into the mammary fat pad yields aggressive tumors that self-renew as evidenced by serial xenograft passages through transplantation. Herein we fully characterize cancer stem cell-like features in 293T human embryonic kidney cells.Results293T cells can be readily cultured and passaged as spheres in serum-free stem cell promoting culture conditions. Cells cultured in vitro as three-dimensional spheres (3D) were shown to contain higher ALDH1 and CD44+/CD24- population compared to monolayer cells. These cells were also resistant to radiation and upregulate stem cell survival signaling including β-catenin, Notch1 and Survivin in response to radiation. Moreover, 3D spheres generated from the 293T cells have increased expression of mesenchymal genes including vimentin, n-cadherin, zeb1, snail and slug as well as pro-metastatic genes RhoC, Tenascin C and MTA1. In addition, microRNAs implicated in self-renewal and metastases were markedly reduced in 3D spheres.Conclusions293T cells exhibit a cancer stem cell-like phenotype when cultured as 3D spheres and represent an important research tool for studying the molecular and biological mechanisms of cancer stem cells and for testing and developing novel targets for cancer therapy.

【 授权许可】

Unknown   
© Debeb et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.