Colorectal cancer derived organotypic spheroids maintain essential tissue characteristics but adapt their metabolism in culture

Proteome Science

Research

Eirik Sundlisaeter¹  Karl Søndenaa²  Reidar Myklebust³  Sébastien Bougnaud⁴  Simone P Niclou⁴  Fred Fack⁴  Uros Rajcevic⁵  Connie R Jiménez⁶  Thang V Pham⁶  Jaco C Knol⁶  Sander Piersma⁶ 

[1]Department of Biomedicine, University of Bergen, Bergen, Norway

[2]Department of Surgery, Haraldsplass Deaconal Hospital, University of Bergen, Bergen, Norway

[3]Haukeland University Hospital, Bergen, Norway

[4]NorLux Neuro-Oncology Laboratory, Department of Oncology, CRP-Santé, Luxembourg, Luxembourg

[5]NorLux Neuro-Oncology Laboratory, Department of Oncology, CRP-Santé, Luxembourg, Luxembourg

[6]Department of Research and Development, Blood Transfusion Center of Slovenia, Ljubljana, Slovenia

[7]Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia

[8]OncoProteomics Laboratory, Department of Medical Oncology, VUmc-Cancer Center Amsterdam, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, the Netherlands

关键词: Colorectal cancer;    Tissue spheroids;    Proteomics;    Mass spectrometry;    Cancer stem cells;    Bioinformatics;    Cancer biomarkers;    Label-free proteomics;    3D cell cultures;   

DOI  :  10.1186/1477-5956-12-39

 received in 2013-10-21, accepted in 2014-06-09,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundOrganotypic tumor spheroids, a 3D in vitro model derived from patient tumor material, preserve tissue heterogeneity and retain structural tissue elements, thus replicating the in vivo tumor more closely than commonly used 2D and 3D cell line models. Such structures harbour tumorigenic cells, as revealed by xenograft implantation studies in animal models and maintain the genetic makeup of the original tumor material. The aim of our work was a morphological and proteomic characterization of organotypic spheroids derived from colorectal cancer tissue in order to get insight into their composition and associated biology.ResultsMorphological analysis showed that spheroids were of about 250 μm in size and varied in structure, while the spheroid cells differed in shape and size and were tightly packed together by desmosomes and tight junctions. Our proteomic data revealed significant alterations in protein expression in organotypic tumor spheroids cultured as primary explants compared to primary colorectal cancer tissue. Components underlying cellular and tissue architecture were changed; nuclear DNA/ chromatin maintenance systems were up-regulated, whereas various mitochondrial components were down-regulated in spheroids. Most interestingly, the mesenchymal cells appear to be substantial component in such cellular assemblies. Thus the observed changes may partly occur in this cellular compartment. Finally, in the proteomics analysis stem cell-like characteristics were observed within the spheroid cellular assembly, reflected by accumulation of Alcam, Ctnnb1, Aldh1, Gpx2, and CD166. These findings were underlined by IHC analysis of Ctnnb1, CD24 and CD44, therefore warranting closer investigation of the tumorigenic compartment in this 3D culture model for tumor tissue.ConclusionsOur analysis of organotypic CRC tumor spheroids has identified biological processes associated with a mixture of cell types and states, including protein markers for mesenchymal and stem-like cells. This 3D tumor model in which tumor heterogeneity is preserved may represent an advantageous model system to investigate novel therapeutic approaches.

【 授权许可】

Unknown   
© Rajcevic et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.