Global diversity and biogeography of DNA viral communities in activated sludge systems

Microbiome

Research

Yang Liu¹  Xinyuan He¹  Zhen Tian¹  Linwei Wu²  Kaili Sun³  Xianghe Zeng³  Rongfeng Gao³  Qiang Li³  Xiangyu Fan⁴  Mengzhi Ji⁵  Dashuai Mu⁶ 

[1] Artificial Intelligence Institute, University of Jinan, Jinan, Shandong Province, China;Institute of Ecology, Key Laboratory for Earth Surface Processes of the Ministry of Education, College of Urban and Environmental Sciences, Peking University, Beijing, China;School of Biological Science and Technology, University of Jinan, Jinan, Shandong Province, China;School of Biological Science and Technology, University of Jinan, Jinan, Shandong Province, China;Artificial Intelligence Institute, University of Jinan, Jinan, Shandong Province, China;School of Biological Science and Technology, University of Jinan, Jinan, Shandong Province, China;Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong Province, China;State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao, Shandong Province, China;Marine College, Shandong University, Weihai, Shandong Province, China;

关键词: Activated sludge;    Viral community;    Biogeography;    Auxiliary metabolic genes;    Virus-microbe interaction;   

DOI  :  10.1186/s40168-023-01672-1

 received in 2023-05-12, accepted in 2023-09-21,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundActivated sludge (AS) systems in wastewater treatment plants (WWTPs) harbor enormous viruses that regulate microbial metabolism and nutrient cycling, significantly influencing the stability of AS systems. However, our knowledge about the diversity of viral taxonomic groups and functional traits in global AS systems is still limited. To address this gap, we investigated the global diversity and biogeography of DNA viral communities in AS systems using 85,114 viral operational taxonomic units (vOTUs) recovered from 144 AS samples collected across 54 WWTPs from 13 different countries.ResultsAS viral communities and their functional traits exhibited distance-decay relationship (DDR) at the global scale and latitudinal diversity gradient (LDG) from equator to mid-latitude. Furthermore, it was observed that AS viral community and functional gene structures were largely driven by the geographic factors and wastewater types, of which the geographic factors were more important. Carrying and disseminating auxiliary metabolic genes (AMGs) associated with the degradation of polysaccharides, sulfate reduction, denitrification, and organic phosphoester hydrolysis, as well as the lysis of crucial functional microbes that govern biogeochemical cycles were two major ways by which viruses could regulate AS functions. It was worth noting that our study revealed a high abundance of antibiotic resistance genes (ARGs) in viral genomes, suggesting that viruses were key reservoirs of ARGs in AS systems.ConclusionsOur results demonstrated the highly diverse taxonomic groups and functional traits of viruses in AS systems. Viral lysis of host microbes and virus-mediated HGT can regulate the biogeochemical and nutrient cycles, thus affecting the performance of AS systems. These findings provide important insights into the viral diversity, function, and ecology in AS systems on a global scale.-K8pwvbs3fYC6bGWQ-q_MyVideo Abstract

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 1

Fig. 2

Fig. 4

12864_2016_3440_Article_IEq6.gif

12951_2015_155_Article_IEq58.gif

12951_2015_155_Article_IEq57.gif

Fig. 1

Fig. 1

Fig. 3

Fig. 5

Fig. 2

Fig. 2

Fig. 4

Fig. 7