Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactisBl-04

BMC Genomics

Research Article

Yong Jun Goh¹  Todd R Klaenhammer¹  Rodolphe Barrangou²  Sampo J Lahtinen³  Birte Svensson⁴  Maher Abou Hachem⁴  Joakim M Andersen⁵ 

[1] Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, 27695, Raleigh, NC, USA;DuPont Nutrition and Health, 3329 Agriculture Drive, 53716, Madison, WI, USA;DuPont Nutrition and Health, Sokeritehtaantie 20, FI-02460, Kantvik, Finland;Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Søltofts Plads Building 224, DK-2800, Kgs. Lyngby, Denmark;Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Søltofts Plads Building 224, DK-2800, Kgs. Lyngby, Denmark;Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, 27695, Raleigh, NC, USA;

关键词: Bifidobacterium lactis;    Transcriptomics;    ABC transporter;    GPH transporter;    Prebiotics;    Glycoside hydrolase;   

DOI  :  10.1186/1471-2164-14-312

 received in 2012-11-13, accepted in 2013-04-18,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundProbiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of α- and β-linked hexoses, and β-xylosides.ResultsThe overall transcriptome was modulated dependent on the type of glycoside (galactosides, glucosides or xylosides) utilized. Carbohydrate transporters of the major facilitator superfamily (induced by gentiobiose and β-galacto-oligosaccharides (GOS)) and ATP-binding cassette (ABC) transporters (upregulated by cellobiose, GOS, isomaltose, maltotriose, melibiose, panose, raffinose, stachyose, xylobiose and β-xylo-oligosaccharides) were differentially upregulated, together with glycoside hydrolases from families 1, 2, 13, 36, 42, 43 and 77. Sequence analysis of the identified solute-binding proteins that determine the specificity of ABC transporters revealed similarities in the breadth and selectivity of prebiotic utilization by bifidobacteria.ConclusionThis study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. Results provide insights into the ability of this probiotic microbe to utilize indigestible carbohydrates in the human gastrointestinal tract.

【 授权许可】

Unknown   
© Andersen et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.