Establishment of a free-mating, long-standing and highly productive laboratory colony of Anopheles darlingi from the Peruvian Amazon

Malaria Journal

Research

Cuauhtémoc Villarreal-Treviño¹  Victor M López-Sifuentes²  Karin Escobedo-Vargas²  Carmen Flores-Mendoza²  Anibal Huayanay-Repetto²  Gissella M Vásquez²  Frederick M Stell²  Andrés G Lescano³  Yvonne-Marie Linton⁴ 

[1] Centro Regional de Investigación en Salud Pública/Instituto Nacional de Salud Pública (CRISP/INSP), Tapachula, Chiapas, Mexico;Department of Entomology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Bellavista, Callao, Peru;Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Bellavista, Callao, Peru;Walter Reed Biosystematics Unit, Museum Support Center, Smithsonian Institution, Suitland, MD, USA;Walter Reed Army Institute of Research, Silver Spring, MD, USA;Department of Entomology, Smithsonian Institution, Washington, DC, USA;

关键词: Anopheles darlingi;    Colony establishment;    Natural copulation induction;    Larval rearing;    Amazon basin;    Peru;    Malaria vector;   

DOI  :  10.1186/s12936-015-0733-0

 received in 2014-12-24, accepted in 2015-05-19,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAnopheles darlingi is the main malaria vector in the Amazon region and is among the most efficient malaria vectors worldwide. However, due to the lack of a well-established laboratory colony, key control-relevant aspects of the bionomics, behaviour, genetics, and vector-parasite relationships of An. darlingi remain unknown. Here, biological parameters that had been successful in initiating other Anopheles colonies were optimized and improved for An. darlingi, with the aim of establish a free-mating, stable, and highly productive laboratory colony.MethodsWild An. darlingi adult females were field collected from Zungarococha, Loreto Department, Peru (03°49′32.40″S, 73°21′00.08″W), and taken to the NAMRU-6 Insectary in Iquitos where F1 offspring were produced and reared. Natural copulation was successfully induced in F1 adults under a thermoperiod of 30 ± 1 °C during the day and 25 ± 1 °C at night, and with a 30-min LED light stimulation period at dusk. Oviposition success was enhanced using egg-laying containers with a dark-coloured surface. Larval feeding regimes were standardized for optimal larval development. Optimized copulation induction methods were used to facilitate mating in An. darlingi until the F10 generation. No copulation induction assistance was needed in subsequent generations.ResultsIn 19 generations, the An. darlingi colony produced a total of 763,775 eggs; 441,124 larvae; 248,041 pupae; and 231,591 adults. A mean of 0.56 sexual encounters/female/cage (n = 36 cages) was recorded across the first ten generations (F1-F10). A mean insemination rate of 54.7 % (n = 5,907 females) ranging from 43.6 % (F2) to 66.6 % (F10) was recorded across nine generations (F2-F10). Free-mating was casually observed in the F8 generation, and subsequently confirmed in the F9 and F10 generations; comparable insemination rates and egg laying between stimulated (51.6 %, 12.9 eggs/female), and non-stimulated (52.3 %, 11.2 eggs/female) females were recorded. The time from egg to adult development ranged from 10 to 20 days. Moreover, the colony was relocated to a new laboratory within Iquitos in the F14 generation without any noted changes in its productivity. By March 2015, the An. darlingi colony has been successfully reared to the F26 generation.ConclusionsThis constitutes the first report of a free-mating, highly productive, and long-standing An. darlingi laboratory colony established through natural copulation induction, which will support critical malaria research. This rearing methodology may be a transferable, cost-effective alternative to labour-intensive forced mating practices widely used in maintaining other Anopheles colonies.

【 授权许可】

Unknown   
© Villarreal-Treviño et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.