Identification of novel MiRNAs and MiRNA expression profiling during grain development in indica rice

BMC Genomics

Research Article

Jiulin Wang¹  Ning Su¹  Xin Zhang¹  Fuqing Wu¹  Xiupin Guo¹  Cailin Lei¹  Zhijun Cheng¹  Long Mao¹  Jie Wang¹  Rongzhi Zhang¹  Yi Shen²  Ling Jiang²  Ying Lan³  Jianmin Wan³ 

[1] National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agriculture Sciences, 100081, Beijing, China;National Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, 210095, Nanjing, China;National Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, 210095, Nanjing, China;National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agriculture Sciences, 100081, Beijing, China;

关键词: miRNA;    Grain filling;    Indica rice;   

DOI  :  10.1186/1471-2164-13-264

 received in 2011-09-13, accepted in 2012-04-03,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) modulate gene expression in different tissues and at diverse developmental stages, including grain development in japonica rice. To identify novel miRNAs in indica rice and to study their expression patterns during the entire grain filling process, small RNAs from all stages of grain development were sequenced and their expression patterns were studied using customized miRNA chips.ResultsA total of 21 conserved and 91 non-conserved miRNA families were found in developing indica grains. We also discovered 11 potential novel miRNAs based on the presence of their miRNA*s. Expression patterns of these identified miRNAs were analyzed using customized miRNA chips. The results showed that during the filling phase about half of the detected miRNAs were up-regulated, whereas the remainder were down-regulated. Predicted targets of differentially expressed miRNAs may participate in carbohydrate metabolism, hormone signaling and pathways associated with seed maturity, suggesting potentially important roles in rice grain development.ConclusionsThis study is the first genome-wide investigation of miRNAs during the grain-filling phase of an indica variety of rice. The novel miRNAs identified might be involved in new miRNA regulatory pathways for grain development. The complexity of these miRNAs and their targets and interactions require further study to obtain a better understanding of the molecular mechanisms underlying grain development.

【 授权许可】

Unknown   
© Lan et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.