Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile

BMC Microbiology

Research Article

Luciano José Pereira¹  Viviam de Oliveira Silva²  Ramiro Mendonça Murata³ 

[1] Department of Health Sciences, Physiology Area, Federal University of Lavras,Lavras, Minas Gerais, Brazil;Herman Ostrow School of Dentistry, Division of Periodontology Diagnostic Sciences, Dental Hygiene & Biomedical Science, University of Southern California, Los Angeles, CA, USA;Department of Veterinary Medicine, Physiology and Pharmacology Area, Federal University of Lavras, Lavras, Minas Gerais, Brazil;School of Dental Medicine, Department Foundational Sciences, East Carolina University, 1851 MacGregor Downs Road, 27834-4354, Greeville, NC, USA;Brody School of Medicine, Department of Microbiology and Immunology, East Carolina University, Greenville, NC, USA;

关键词: Aggregatibacter actinomycetemcomitans;    Periodontal disease;    Host response;    Keratinocyte;    Fibroblast;    Immunomodulation;   

DOI  :  10.1186/s12866-017-0946-1

 received in 2016-10-30, accepted in 2017-02-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/mL or 20 μg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant.ResultsThe relative expression of interleukin (IL)-1-α and IL-18 genes in HGF-1 decreased with 10 μg/mL or 20 μg/mL of β-glucan, where as the expression of PTGS-2 decreased only with 10 μg/mL. The expression of IL-1-α increased with 20 μg/mL and that of IL-18 increased with 10 μg/mL in OBA-9; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of β-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 μg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 μg/mL of β-glucan.ConclusionsTreatment with β-glucans positively modulated the immune response and production of metabolites.

【 授权许可】

CC BY   
© The Author(s). 2017