Critical role of leukotriene B4 receptor signaling in mouse 3T3-L1 preadipocyte differentiation

Lipids in Health and Disease

Research

Takashi Yamashiro¹  Yuka Murata²  Kae Hirata²  Yoshinori Kamisaki³  Koichiro Wada³  Atsushi Nakajima⁴ 

[1] Department of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Osaka University, Suita, 565-0871, Osaka, Japan;Department of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Osaka University, Suita, 565-0871, Osaka, Japan;Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, 565-0871, Osaka, Japan;Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, 565-0871, Osaka, Japan;Gastroenterology Division, Yokohama City University School of Medicine, 236-0004, Yokohama, Kanagawa, Japan;

关键词: Leukotrienes;    Preadipocyte differentiation;    Mouse 3T3-L1 fibroblasts;    BLT;    siRNA;   

DOI  :  10.1186/1476-511X-12-122

 received in 2013-07-05, accepted in 2013-08-06,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundVarious inflammatory mediators related to obesity might be closely related to insulin resistance. Leukotrienes (LTs) are involved in inflammatory reactions. However, there are few reports regarding the role of LTs in adipocyte differentiation. Therefore, we investigated the role of leukotriene B4 (LTB4)-leukotriene receptor (BLT) signaling in mouse 3T3-L1 fibroblastic preadipocyte differentiation to mature adipocytes.MethodsMouse 3T3-L1 preadipocytes were treated with lipoxygenase (LOX) inhibitors, BLT antagonist, and small interfering RNA (siRNA) for BLT1 and BLT2 to block the LTB4-BLT signaling pathway, then the adipocyte differentiation such as lipid accumulation and the increase in triglyceride was evaluated.ResultsBlockade of BLT signaling by treatment with a LOX inhibitor or a BLT antagonist suppressed preadipocyte differentiation into mature adipocytes. In addition, knockdown of BLT1 and BLT2 by siRNAs dramatically inhibited differentiation. These results indicate the LTB4-BLT signaling pathway may positively regulate preadipocyte differentiation and be a rate-limiting system to control adipocyte differentiation.ConclusionsThe LTB4-BLT signaling pathway provides a potent regulatory signal that accelerates the differentiation of mouse 3T3-L1 preadipocytes. Further investigations are necessary to confirm the exact role of LTB4 and BLTs signaling pathways in preadipocyte differentiation.

【 授权许可】

CC BY   
© Hirata et al.; licensee BioMed Central Ltd. 2013