Methyl jasmonate abolishes the migration, invasion and angiogenesis of gastric cancer cells through down-regulation of matrix metalloproteinase 14

BMC Cancer

Research Article

Kai Huang¹  Qiangsong Tong²  Ling Tong³  Liduan Zheng⁴  Xuan Xiang⁵  Meng Qi⁵  Jiarui Pu⁵  Dan Li⁵ 

[1] Clinical Center of Human Genomic Research, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;Department of Cardiology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;Clinical Center of Human Genomic Research, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;Department of Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;Clinical Center of Human Genomic Research, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;Department of Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People's Republic of China;

关键词: Gastric cancer;    Methyl jasmonate;    Matrix metalloproteinase 14;    Specificity protein 1;   

DOI  :  10.1186/1471-2407-13-74

 received in 2012-09-17, accepted in 2013-01-30,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundRecent evidence indicates that methyl jasmonate (MJ), a plant stress hormone, exhibits anti-cancer activity on human cancer cells. The aim of this study is to determine whether sub-cytotoxic MJ can abolish the migration, invasion and angiogenesis gastric cancer cells.MethodsHuman gastric cancer cell lines SGC-7901 and MKN-45 were treated with diverse concentrations of MJ. Cell viability, proliferation, migration, invasion and angiogenesis capabilities of cancer cells were measured by MTT colorimetry, EdU incorporation, scratch assay, matrigel invasion assay, and tube formation assay. Gene expression was detected by western blot and real-time quantitative RT-PCR. Binding of transcription factor on gene promoter was detected by chromatin immunoprecipitation.ResultsSub-cytotoxic (0.05 to 0.2 mM) MJ attenuated the migration, invasion and angiogenesis, but not the cell viability or proliferation, of gastric cancer cells in a time- and dose-dependent manner, with down-regulation of matrix metalloproteinase 14 (MMP-14) and its downstream gene vascular endothelial growth factor. Restoration of MMP-14 expression rescued the SGC-7901 and MKN-45 cells from sub-cytotoxic MJ-inhibited migration, invasion and angiogenesis. In addition, sub-cytotoxic MJ decreased the specificity protein 1 (Sp1) expression and binding on MMP-14 promoter, while restoration of Sp1 expression rescued the cancer cells from sub-cytotoxic MJ-mediated defects in MMP-14 expression, migration, invasion and angiogenesis.ConclusionsSub-cytotoxic MJ attenuates the MMP-14 expression via decreasing the Sp1 expression and binding on MMP-14 promoter, thus inhibiting the migration, invasion and angiogenesis of gastric cancer cells.

【 授权许可】

Unknown   
© Zheng et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.