Construction of energy-conserving sucrose utilization pathways for improving poly-γ-glutamic acid production in Bacillus amyloliquefaciens

Microbial Cell Factories

Research

Yi Wang¹  Mingfeng Cao²  Xiaoyun Lu³  Weixia Gao⁴  Cunjiang Song⁴  Yufen Quan⁴  Yulei Dang⁴  Yanyan Gu⁵  Jun Feng⁶  Shufang Wang⁷ 

[1] Department of Biosystems Engineering, Auburn University, 36849, Auburn, AL, USA;Department of Chemical and Biological Engineering, Iowa State University, 50011, Ames, IA, USA;Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, 710049, Xi’an, Shaanxi, China;Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, 300071, Tianjin, China;Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, 300071, Tianjin, China;Department of Biosystems Engineering, Auburn University, 36849, Auburn, AL, USA;Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, 300071, Tianjin, China;Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, 710049, Xi’an, Shaanxi, China;Department of Biosystems Engineering, Auburn University, 36849, Auburn, AL, USA;State Key Laboratory of Medicinal Chemical Biology, Nankai University, 94 Weijin Road, 300071, Tianjin, China;State Key Laboratory of Medicinal Chemical Biology, Nankai University, 94 Weijin Road, 300071, Tianjin, China;

关键词: Bacillus amyloliquefaciens;    Sucrose utilization pathway;    Phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS);    Energy-conserving pathway;    Sucrose permease;    Sucrose phosphorylase;    Poly-γ-glutamic acid (γ-PGA);   

DOI  :  10.1186/s12934-017-0712-y

 received in 2017-01-28, accepted in 2017-06-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSucrose is an naturally abundant and easily fermentable feedstock for various biochemical production processes. By now, several sucrose utilization pathways have been identified and characterized. Among them, the pathway consists of sucrose permease and sucrose phosphorylase is an energy-conserving sucrose utilization pathway because it consumes less ATP when comparing to other known pathways. Bacillus amyloliquefaciens NK-1 strain can use sucrose as the feedstock to produce poly-γ-glutamic acid (γ-PGA), a highly valuable biopolymer. The native sucrose utilization pathway in NK-1 strain consists of phosphoenolpyruvate-dependent phosphotransferase system and sucrose-6-P hydrolase and consumes more ATP than the energy-conserving sucrose utilization pathway.ResultsIn this study, the native sucrose utilization pathway in NK-1 was firstly deleted and generated the B. amyloliquefaciens 3Δ strain. Then four combination of heterologous energy-conserving sucrose utilization pathways were constructed and introduced into the 3Δ strain. Results demonstrated that the combination of cscB (encodes sucrose permease) from Escherichia coli and sucP (encodes sucrose phosphorylase) from Bifidobacterium adolescentis showed the highest sucrose metabolic efficiency. The corresponding mutant consumed 49.4% more sucrose and produced 38.5% more γ-PGA than the NK-1 strain under the same fermentation conditions.ConclusionsTo our best knowledge, this is the first report concerning the enhancement of the target product production by introducing the heterologous energy-conserving sucrose utilization pathways. Such a strategy can be easily extended to other microorganism hosts for reinforced biochemical production using sucrose as substrate.

【 授权许可】

CC BY   
© The Author(s) 2017