Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters

Microbial Cell Factories

Review

Norma A Valdez-Cruz¹  Octavio T Ramírez¹  Luis Caspeta¹  Néstor O Pérez²  Mauricio A Trujillo-Roldán³ 

[1]Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México. Avenida Universidad, 2001, Cuernavaca Morelos, México

[2]Probiomed S.A. de C.V. Planta Tenancingo, Cruce de Carreteras Acatzingo-Zumpahuacan SN, Tenancingo, Edo., C.P. 52400, de México, México

[3]Unidad de Bioprocesos, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228 D.F., C.P 04510, México, México

关键词: Recombinant Protein;    Specific Growth Rate;    Heterologous Protein;    Recombinant Protein Production;    Heterologous Protein Expression;   

DOI  :  10.1186/1475-2859-9-18

 received in 2010-01-27, accepted in 2010-03-19,  发布年份 2010

来源: Springer

【 摘 要 】

The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use to produce recombinant proteins in prokariotic cells. In this expression system, induction of heterologous protein is achieved by increasing the culture temperature, generally above 37°C. Concomitant to the overexpression of heterologous protein, the increase in temperature also causes a variety of complex stress responses. Many studies have reported the use of such temperature inducible expression system, however only few discuss the simultaneous stress effects caused by recombinant protein production and the up-shift in temperature. Understanding the integral effect of such responses should be useful to develop improved strategies for high yield protein production and recovery. Here, we describe the current status of the heat inducible expression system based on the pL and/or pR λ phage promoters, focusing on recent developments on expression vehicles, the stress responses at the molecular and physiological level that occur after heat induction, and bioprocessing factors that affect protein overexpression, including culture operation variables and induction strategies.

【 授权许可】

Unknown   
© Valdez-Cruz et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.