Elevation of sulfatides in ovarian cancer: An integrated transcriptomic and lipidomic analysis including tissue-imaging mass spectrometry

Molecular Cancer

Research

Ying Liu¹  John F McDonald¹  Elaine Wang¹  L DeEtte Walker¹  Amin Momin¹  Nathan J Bowen¹  Lilya V Matyunina¹  M Cameron Sullards²  Alfred H Merrill²  Yanfeng Chen³  Rebecca Shaner³ 

[1] School of Biology and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, 315 Ferst Drive, 30332-0363, Atlanta, Georgia, USA;School of Biology and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, 315 Ferst Drive, 30332-0363, Atlanta, Georgia, USA;School of Chemistry and Biochemistry and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, 315 Ferst Drive, 30332-0363, Atlanta, Georgia, USA;School of Chemistry and Biochemistry and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, 315 Ferst Drive, 30332-0363, Atlanta, Georgia, USA;

关键词: Ovarian Cancer;    Ovarian Carcinoma;    Laser Capture Microdissection;    Ovarian Epithelial Carcinoma;    Sphingoid Base;   

DOI  :  10.1186/1476-4598-9-186

 received in 2010-02-02, accepted in 2010-07-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSulfatides (ST) are a category of sulfated galactosylceramides (GalCer) that are elevated in many types of cancer including, possibly, ovarian cancer. Previous evidence for elevation of ST in ovarian cancer was based on a colorimetric reagent that does not provide structural details and can also react with other lipids. Therefore, this study utilized mass spectrometry for a structure-specific and quantitative analysis of the types, amounts, and tissue localization of ST in ovarian cancer, and combined these findings with analysis of mRNAs for the relevant enzymes of ST metabolism to explore possible mechanisms.ResultsAnalysis of 12 ovarian tissues graded as histologically normal or having epithelial ovarian tumors by liquid chromatography electrospray ionization-tandem mass spectrometry (LC ESI-MS/MS) established that most tumor-bearing tissues have higher amounts of ST. Because ovarian cancer tissues are comprised of many different cell types, histological tissue slices were analyzed by matrix-assisted laser desorption ionization-tissue-imaging MS (MALDI-TIMS). The regions where ST were detected by MALDI-TIMS overlapped with the ovarian epithelial carcinoma as identified by H & E staining and histological scoring. Furthermore, the structures for the most prevalent species observed via MALDI-TIMS (d18:1/C16:0-, d18:1/C24:1- and d18:1/C24:0-ST) were confirmed by MALDI-TIMS/MS, whereas, a neighboring ion(m/z 885.6) that was not tumor specific was identified as a phosphatidylinositol. Microarray analysis of mRNAs collected using laser capture microdissection revealed that expression of GalCer synthase and Gal3ST1 (3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase) were approximately 11- and 3.5-fold higher, respectively, in the ovarian epithelial carcinoma cells versus normal ovarian stromal tissue, and they were 5- and 2.3-fold higher in comparison with normal surface ovarian epithelial cells, which is a likely explanation for the higher ST.ConclusionsThis study combined transcriptomic and lipidomic approaches to establish that sulfatides are elevated in ovarian cancer and should be evaluated further as factors that might be important in ovarian cancer biology and, possibly, as biomarkers.

【 授权许可】

Unknown   
© Liu et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.