The murine lung microbiome in relation to the intestinal and vaginal bacterial communities

BMC Microbiology

Research Article

Lars Hestbjerg Hansen¹  Søren Johannes Sørensen¹  Michael Roggenbuck¹  Søren Thor Larsen²  Susanne Schjørring³  Karen Angeliki Krogfelt³  Kenneth Klingenberg Barfod⁴ 

[1] Department of Biology, Microbiology, University of Copenhagen, Universitetsparken 15, 2100, Copenhagen O, Denmark;National Research Centre for the Working Environment, Lersø Parkallé 105, 2100, Copenhagen O, Denmark;Statens Serum Institut, Artillerivej 5Copenhagen S, 2300, Denmark;Statens Serum Institut, Artillerivej 5Copenhagen S, 2300, Denmark;National Research Centre for the Working Environment, Lersø Parkallé 105, 2100, Copenhagen O, Denmark;

关键词: Operational Taxonomic Unit;    Lung Sample;    Lung Tissue Sample;    Vaginal Microbiota;    Inflammatory Lung Disease;   

DOI  :  10.1186/1471-2180-13-303

 received in 2013-07-18, accepted in 2013-12-23,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThis work provides the first description of the bacterial population of the lung microbiota in mice. The aim of this study was to examine the lung microbiome in mice, the most used animal model for inflammatory lung diseases such as COPD, cystic fibrosis and asthma.Bacterial communities from broncho-alveolar lavage fluids and lung tissue were compared to samples taken from fecal matter (caecum) and vaginal lavage fluid from female BALB/cJ mice.ResultsUsing a customized 16S rRNA sequencing protocol amplifying the V3-V4 region our study shows that the mice have a lung microbiome that cluster separately from mouse intestinal microbiome (caecum). The mouse lung microbiome is dominated by Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Cyanobacteria overlapping the vaginal microbiome. We also show that removal of host tissue or cells from lung fluid during the DNA extraction step has an impact on the resulting bacterial community profile. Sample preparation needs to be considered when choosing an extraction method and interpreting data.ConclusionsWe have consistently amplified bacterial DNA from mouse lungs that is distinct from the intestinal microbiome in these mice. The gut microbiome has been extensively studied for its links to development of disease. Here we suggest that also the lung microbiome could be important in relation to inflammatory lung diseases. Further research is needed to understand the contribution of the lung microbiome and the gut-lung axis to the development of lung diseases such as COPD and asthma.

【 授权许可】

CC BY   
© Barfod et al.; licensee BioMed Central Ltd. 2013