Antifungal activity of pomegranate peel extract and isolated compound punicalagin against dermatophytes

Annals of Clinical Microbiology and Antimicrobials

Research

Diógenes AG Cortez¹  Celso V Nakamura²  Benedito P Dias Filho²  Tania Ueda-Nakamura²  Simone R Foss³  Eliana H Endo⁴ 

[1] Departament of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil;Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil;Post graduation in Microbiology, Universidade Estadual de Londrina, Londrina, PR, Brazil;Post graduation in Pharmaceutical Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil;

关键词: Crude Extract;    Conidial Germination;    Dermatophytosis;    Hydrolyzable Tannin;    Hydroalcoholic Extract;   

DOI  :  10.1186/s12941-014-0032-6

 received in 2013-12-13, accepted in 2014-07-13,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDermatophyte species infect the epidermis and appendages, often with serious social and health-economic consequences. The hydroalcoholic extract of pomegranate fruit peel showed activity against the dermatophyte fungi Trichophyton mentagrophytes, T. rubrum, Microsporum canis and M. gypseum.MethodsHydroalcoholic extract was prepared with pomegranate peels. This crude extract was fractionated and submitted to liquid-liquid partition, resulting in an active fraction which was fractionated in a Sephadex LH-20 column, followed by a Lobar column. The structure of the active compound was established with the use of spectroscopic methods.ResultsThe crude extract of pomegranate fruit peel showed activity against the dermatophytes Trichophyton mentagrophytes, T. rubrum, Microsporum canis, and M. gypseum, with MICs values of 125μg/ml and 250μg/ml, respectively for each genus. Punicalagin was isolated and identified by spectroscopic analysis. The crude extract and punicalagin showed activity against the conidial and hyphal stages of the fungi. The cytotoxicity assay showed selectivity for fungal cells than for mammalian cells.ConclusionsThese results indicated that the crude extract and punicalagin had a greater antifungal activity against T. rubrum, indicating that the pomegranate is a good target for study to obtain a new antidermatophyte medicine.

【 授权许可】

Unknown   
© Foss et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.