Reversine suppresses oral squamous cell carcinoma via cell cycle arrest and concomitantly apoptosis and autophagy

Journal of Biomedical Science

Research

Jeff Yi-Fu Chen¹  Wei-Ching Wu²  Ya-Ping Cheng²  Wen-Tsai Ji²  Hau-Ren Chen²  Ming-Ko Chiang²  Ying-Ray Lee³ 

[1] Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Life Science, Institute of Molecular Biology and Institute of Biomedical Science, College of Science, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan;Department of Medical Research, Chiayi Christian Hospital, Chia-Yi, Taiwan;Department of Nursing, Min-Hwei College of Health Care Management, Tainan, Taiwan;

关键词: Reversine;    cell cycle arrest;    apoptosis;    autophagy;    oral squamous cell carcinoma (OSCC);   

DOI  :  10.1186/1423-0127-19-9

 received in 2011-12-21, accepted in 2012-01-27,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe effective therapies for oral cancer patients of stage III and IV are generally surgical excision and radiation combined with adjuvant chemotherapy using 5-Fu and Cisplatin. However, the five-year survival rate is still less than 30% in Taiwan. Therefore, evaluation of effective drugs for oral cancer treatment is an important issue. Many studies indicated that aurora kinases (A, B and C) were potential targets for cancer therapies. Reversine was proved to be a novel aurora kinases inhibitor with lower toxicity recently. In this study, the potentiality for reversine as an anticancer agent in oral squamous cell carcinoma (OSCC) was evaluated.MethodsEffects of reversine on cell growth, cell cycle progress, apoptosis, and autophagy were evaluated mainly by cell counting, flow cytometry, immunoblot, and immunofluorescence.ResultsThe results demonstrated that reversine significantly suppressed the proliferation of two OSCC cell lines (OC2 and OCSL) and markedly rendered cell cycle arrest at G2/M stage. Reversine also induced cell death via both caspase-dependent and -independent apoptosis. In addition, reversine could inhibit Akt/mTORC1 signaling pathway, accounting for its ability to induce autophagy.ConclusionsTaken together, reversine suppresses growth of OSCC via multiple mechanisms, which may be a unique advantage for developing novel therapeutic regimens for treatment of oral cancer in the future.

【 授权许可】

Unknown   
© Lee et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.