The beneficial fungus Piriformospora indica protects Arabidopsis from Verticillium dahliaeinfection by downregulation plant defense responses

BMC Plant Biology

Research Article

Sheqin Dong¹  Binggan Lou²  Kai-Wun Yeh³  Chao Sun⁴  Irena Sherameti⁴  Ralf Oelmüller⁴  Khabat Vahabi⁴  Samik Bhattacharya⁵  Yongqi Shao⁵  Ian T Baldwin⁵  Jing Lu⁶ 

[1] College of Life Sciences, Yangtze University, Jingzhou, China;Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, China;Institute of Plant Biology, National Taiwan University, Taipei, Taiwan;Institute of Plant Physiology, Friedrich-Schiller-University Jena, Dornburger Str. 159, 07743, Jena, Germany;Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, D-07745, Jena, Germany;Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, D-07745, Jena, Germany;Institute of Insect Sciences, Zhejiang University, 310058, Hangzhou, China;

关键词: Calcium;    Defense;    Ethylene;    Jasmonic acid;    Piriformospora indica;    Salicylic acid;    Verticillium dahliae;   

DOI  :  10.1186/s12870-014-0268-5

 received in 2014-07-02, accepted in 2014-09-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundVerticillium dahliae (Vd) is a soil-borne vascular pathogen which causes severe wilt symptoms in a wide range of plants. The microsclerotia produced by the pathogen survive in soil for more than 15 years.ResultsHere we demonstrate that an exudate preparation induces cytoplasmic calcium elevation in Arabidopsis roots, and the disease development requires the ethylene-activated transcription factor EIN3. Furthermore, the beneficial endophytic fungus Piriformospora indica (Pi) significantly reduced Vd-mediated disease development in Arabidopsis. Pi inhibited the growth of Vd in a dual culture on PDA agar plates and pretreatment of Arabidopsis roots with Pi protected plants from Vd infection. The Pi-pretreated plants grew better after Vd infection and the production of Vd microsclerotia was dramatically reduced, all without activating stress hormones and defense genes in the host.ConclusionsWe conclude that Pi is an efficient biocontrol agent that protects Arabidopsis from Vd infection. Our data demonstrate that Vd growth is restricted in the presence of Pi and the additional signals from Pi must participate in the regulation of the immune response against Vd.

【 授权许可】

Unknown   
© Sun et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.