Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach

BMC Genomics

Research Article

Lu Qin¹  Changbing Yu¹  Xiangsheng Liao¹  Xiaojia Hu¹  Yinshui Li¹  Lihua Xie¹  Yangmin Gong¹  Lei Hu¹  Xing Liao¹  Jude E. Maul²  Daniel P. Roberts²  Dilip K. Lakshman² 

[1] Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 430062, Wuhan, People’s Republic of China;Sustainable Agricultural Systems Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, USDA-Agricultural Research Service, 20705-2350, Beltsville, MD, USA;

关键词: Aspergillus;    Gene expression;    Mycoparasitism;    Proteomics;    Sclerotinia sclerotiorum;   

DOI  :  10.1186/s12864-017-4016-8

 received in 2017-04-12, accepted in 2017-08-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe biological control agent Aspergillus aculeatus Asp-4 colonizes and degrades sclerotia of Sclerotinia sclerotiorum resulting in reduced germination and disease caused by this important plant pathogen. Molecular mechanisms of mycoparasites underlying colonization, degradation, and reduction of germination of sclerotia of this and other important plant pathogens remain poorly understood.ResultsAn RNA-Seq screen of Asp-4 growing on autoclaved, ground sclerotia of S. sclerotiorum for 48 h identified 997 up-regulated and 777 down-regulated genes relative to this mycoparasite growing on potato dextrose agar (PDA) for 48 h. qRT-PCR time course experiments characterized expression dynamics of select genes encoding enzymes functioning in degradation of sclerotial components and management of environmental conditions, including environmental stress. This analysis suggested co-temporal up-regulation of genes functioning in these two processes. Proteomic analysis of Asp-4 growing on this sclerotial material for 48 h identified 26 up-regulated and 6 down-regulated proteins relative to the PDA control. Certain proteins with increased abundance had putative functions in degradation of polymeric components of sclerotia and the mitigation of environmental stress.ConclusionsOur results suggest co-temporal up-regulation of genes involved in degradation of sclerotial compounds and mitigation of environmental stress. This study furthers the analysis of mycoparasitism of sclerotial pathogens by providing the basis for molecular characterization of a previously uncharacterized mycoparasite-sclerotial interaction.

【 授权许可】

CC BY   
© The Author(s). 2017