Functional proteomic analysis of seminal plasma proteins in men with various semen parameters

Reproductive Biology and Endocrinology

Research

Banu Gopalan¹  Rachel Jesudasan²  Edmund Sabanegh³  Rakesh Sharma³  Ashok Agarwal³  Gayatri Mohanty⁴  Satya P Yadav⁵  Belinda Willard⁶ 

[1] Bioinformatics Core Services, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;Center for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India;Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA;Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA;Permanent Address: Ravenshaw University, Cuttack, Odisha, India;Molecular Biotechnology Core lab, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;Proteomics Core Services, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA;

关键词: Seminal Plasma;    Protein Tyrosine Phosphatase;    Male Infertility;    Prostatic Acid Phosphatase;    Prolactin Induce Protein;   

DOI  :  10.1186/1477-7827-11-38

 received in 2013-01-15, accepted in 2013-03-22,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAlterations at the molecular level in spermatozoa and seminal plasma can affect male fertility. The objective of this study was to determine if analysis of differential expression of proteins in varying semen parameters can serve as potential biomarkers for male infertility.MethodsThe differential expression of proteins in the seminal plasma of men based on sperm count and morphology were examined utilizing proteomic tools. Subjects were categorized based on sperm concentration and morphology into 4 groups: 1) normal sperm count and normal morphology (NN); 2) normal sperm count and abnormal morphology (NA); 3) oligozoospermia and normal morphology (ON); and 4) oligozoospermia and abnormal morphology (OA). Proteomic analysis was performed by LC-MS/MS followed by functional bioinformatics analysis. Protein distribution in the NA, ON and OA groups was compared with that of the NN group.ResultsTwenty proteins were differentially expressed among the 4 groups. Among the unique proteins identified, 3 were downregulated in the NA group, 1 in the ON group and 1 in the OA group while 2 were upregulated in the ON and OA groups. The functional analysis 1) identified biological regulation as the major processes affected and 2) determined that most of the identified proteins were of extracellular origin.ConclusionsWe have identified proteins that are over-or underexpressed in the seminal plasma of men with poor sperm quality. The distinct presence of some of the proteins may serve as potential biomarkers and provide insight into the mechanistic role played by these proteins in male infertility. Further studies using Western Blot analysis are required to validate these findings.

【 授权许可】

Unknown   
© Sharma et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.