Identification and evaluation of the role of the manganese efflux protein in Deinococcus radiodurans

BMC Microbiology

Research Article

Hongdan Zhan¹  Guangzhi Xu¹  Zongtao Sun¹  Bing Tian¹  Hongxing Sun¹  Yuejin Hua¹  Huan Chen² 

[1] Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, 310029, Hangzhou, PR, China;Zhejiang Institute of Microbiology, Zhejiang Province, 310012, Hangzhou, PR, China;

关键词: Manganese;    Ionize Radiation;    Radiation Resistance;    Deinococcus Radiodurans;    Cation Diffusion Facilitator;   

DOI  :  10.1186/1471-2180-10-319

 received in 2010-06-09, accepted in 2010-12-14,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundDeinococcus radiodurans accumulates high levels of manganese ions, and this is believed to be correlated with the radiation resistance ability of this microorganism. However, the maintenance of manganese ion homeostasis in D. radiodurans remains to be investigated.ResultsIn this study, we identified the manganese efflux protein (MntE) in D. radiodurans. The null mutant of mntE was more sensitive than the wild-type strain to manganese ions, and the growth of the mntE mutant was delayed in manganese-supplemented media. Furthermore, there was a substantial increase in the in vivo concentration of manganese ions. Consistent with these characteristics, the mntE mutant was more resistant to H2O2, ultraviolet rays, and γ-radiation. The intracellular protein oxidation (carbonylation) level of the mutant strain was remarkably lower than that of the wild-type strain.ConclusionsOur results indicated that dr1236 is indeed a mntE homologue and is indispensable for maintaining manganese homeostasis in D. radiodurans. The data also provide additional evidence for the involvement of intracellular manganese ions in the radiation resistance of D. radiodurans.

【 授权许可】

Unknown   
© Sun et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.