Comparative analysis of the Spirulina platensis subcellular proteome in response to low- and high-temperature stresses: uncovering cross-talk of signaling components

Proteome Science

Research

Sittiruk Roytrakul¹  Apiradee Hongsthong¹  Pavinee Kurdrid¹  Jittisak Senachak¹  Matura Sirijuntarut²  Rayakorn Yutthanasirikul²  Phuttawadee Phuengcharoen²  Wattana Jeamton²  Supapon Cheevadhanarak³ 

[1] BEC Unit, National Center for Genetic Engineering and Biotechnology, 49 Soi Theintalay 25, Thakham, 10150, Bangkok, Bangkhuntien, Thailand;Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Theintalay 25, Thakham, 10150, Bangkok, Bangkhuntien, Thailand;School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, 49 Soi Theintalay 25, Thakham, 10150, Bangkok, Bangkhuntien, Thailand;

关键词: Temperature Stress;    Subcellular Fraction;    Spirulina;    Protein Expression Pattern;    Ammonia Assimilation;   

DOI  :  10.1186/1477-5956-9-39

 received in 2011-01-07, accepted in 2011-07-15,  发布年份 2011

来源: Springer

【 摘 要 】

The present study focused on comparative proteome analyses of low- and high-temperature stresses and potential protein-protein interaction networks, constructed by using a bioinformatics approach, in response to both stress conditions.The data revealed two important points: first, the results indicate that low-temperature stress is tightly linked with oxidative stress as well as photosynthesis; however, no specific mechanism is revealed in the case of the high-temperature stress response. Second, temperature stress was revealed to be linked with nitrogen and ammonia assimilation. Moreover, the data also highlighted the cross-talk of signaling pathways. Some of the detected signaling proteins, e.g., Hik14, Hik26 and Hik28, have potential interactions with differentially expressed proteins identified in both temperature stress conditions. Some differentially expressed proteins found in the Spirulina protein-protein interaction network were also examined for their physical interactions by a yeast two hybrid system (Y2H). The Y2H results obtained in this study suggests that the potential PPI network gives quite reliable potential interactions for Spirulina. Therefore, the bioinformatics approach employed in this study helps in the analysis of phenomena where proteome analyses of knockout mutants have not been carried out to directly examine for specificity or cross-talk of signaling components.

【 授权许可】

Unknown   
© Kurdrid et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.