Chronic kidney disease-related atherosclerosis - proteomic studies of blood plasma

Proteome Science

Research

Dorota Formanowicz¹  Andrzej Wykretowicz²  Elzbieta Pawliczak³  Maria Wanic-Kossowska³  Magdalena Luczak⁴  Marek Figlerowicz⁵ 

[1] Department of Clinical Biochemistry, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780, Poznan, Poland;Department of Internal Medicine, Division of Cardiology - Intensive Therapy, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland;Department of Nephrology, Transplantology and Internal Medicine, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland;Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland;Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland;Institute of Computing Science, Poznan University of Technology, Piotrowo 3A, 60-965, Poznan, Poland;

关键词: Chronic Kidney Disease;    Chronic Kidney Disease Patient;    Chronic Kidney Disease Progression;    Comparative Proteomic Analysis;    Chronic Kidney Disease Group;   

DOI  :  10.1186/1477-5956-9-25

 received in 2011-01-21, accepted in 2011-05-13,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundAtherosclerosis is considered the major cause of the dramatic increase in cardiovascular mortality among patients suffering from chronic kidney disease (CKD). Although the close connection between atherosclerosis and kidney dysfunction is undeniable, factors enhancing CKD-mediated plaque formation are still not well recognized.ResultsTo increase our knowledge of this process we carried out a comparative proteomic analysis of blood plasma proteins isolated from 75 patients in various stages of renal dysfunction (CKD group), 25 patients with advanced cardiovascular disease (CVD group) and 25 healthy volunteers (HV group). The collected samples were subjected to 2D electrophoresis. Then, individual proteins were identified by mass spectrometry. The comparative analysis involving CKD and HV groups showed a differential accumulation of α-1-microglobulin, apolipoprotein A-IV, γ-fibrinogen and haptoglobin in patients with kidney disease. Exactly the same proteins were identified as differentially expressed when proteomes of CVD patients and HV were compared. However, a direct comparison of CKD and CVD groups revealed significant differences in the accumulation of two proteins: α-1-microglobulin and apolipoprotein A-IV.ConclusionsThe obtained results indicate that at least two processes differentially contribute to the plaque formation in CKD- and CVD-mediated atherosclerosis. It seems that the inflammatory process is more intense in CKD patients. On the other hand, the down- and up-regulation of apolipoprotein A-IV in CVD and CKD groups, respectively, suggests that substantial differences exist in the efficacy of cholesterol transport in both groups of patients.

【 授权许可】

Unknown   
© Luczak et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.