Microbial Cell Factories | |
Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process | |
Review | |
Arun Kumar Upadhyay1  Vaibhav Upadhyay1  Surinder Mohan Singh1  Amulya Kumar Panda1  Anupam Singh1  | |
[1] Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, 110067, New Delhi, India; | |
关键词: Inclusion body; Recombinant protein; Solubilization; Refolding; | |
DOI : 10.1186/s12934-015-0222-8 | |
received in 2014-12-15, accepted in 2015-03-06, 发布年份 2015 | |
来源: Springer | |
【 摘 要 】
Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion bodies is labor intensive and the yields of recombinant protein are often low. Here we review the developments in the field that are targeted at improving the yield, as well as quality of the recombinant protein by optimizing the individual steps of the process, especially solubilization of the inclusion bodies and refolding of the solubilized protein. Mild solubilization methods have been discussed which are based on the understanding of the fact that protein molecules in inclusion body aggregates have native-like structure. These methods solubilize the inclusion body aggregates while preserving the native-like protein structure. Subsequent protein refolding and purification results in high recovery of bioactive protein. Other parameters which influence the overall recovery of bioactive protein from inclusion bodies have also been discussed. A schematic model describing the utility of mild solubilization methods for high throughput recovery of bioactive protein has also been presented.
【 授权许可】
Unknown
© Singh et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
【 预 览 】
Files | Size | Format | View |
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RO202311105335686ZK.pdf | 790KB | download |
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