Barrier screens: a method to sample blood-fed and host-seeking exophilic mosquitoes

Malaria Journal

Methodology

Robert D Cooper¹  Frank H Collins²  Neil F Lobo²  Supraman Sukawati³  Hugo Bugoro⁴  Lisa J Reimer⁵  Tanya L Russell⁶  Thomas R Burkot⁷  Nigel W Beebe⁸ 

[1] Australian Army Malaria Institute, Gallipoli Barracks, 4051, Enoggera, QLD, Australia;Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, IN, USA;National Institute of Health, Research and Development, Health Ecology Research and Development Center, Jakarta, Indonesia;National Vector Borne Disease Control Programme, Ministry of Health, Honiara, Solomon Islands;Papua New Guinea Institute of Medical Research, Madang, Papua New Guinea;Case Western Reserve University, 44106, Cleveland, OH, USA;Queensland Tropical Health Alliance, James Cook University, 4870, Cairns, QLD, Australia;Queensland Tropical Health Alliance, James Cook University, 4870, Cairns, QLD, Australia;Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, 30333, Atlanta, GA, USA;School of Biological Sciences, University of Queensland, 4068, St. Lucia, QLD, Australia;CSIRO Ecosystem Sciences, Dutton Park, QLD4102, Brisbane, Australia;

关键词: Mosquito sampling;    Human blood index;    Barrier screen trap;    Exophily;   

DOI  :  10.1186/1475-2875-12-49

 received in 2012-12-05, accepted in 2013-01-31,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundDetermining the proportion of blood meals on humans by outdoor-feeding and resting mosquitoes is challenging. This is largely due to the difficulty of finding an adequate and unbiased sample of resting, engorged mosquitoes to enable the identification of host blood meal sources. This is particularly difficult in the south-west Pacific countries of Indonesia, the Solomon Islands and Papua New Guinea where thick vegetation constitutes the primary resting sites for the exophilic mosquitoes that are the primary malaria and filariasis vectors.MethodsBarrier screens of shade-cloth netting attached to bamboo poles were constructed between villages and likely areas where mosquitoes might seek blood meals or rest. Flying mosquitoes, obstructed by the barrier screens, would temporarily stop and could then be captured by aspiration at hourly intervals throughout the night.ResultsIn the three countries where this method was evaluated, blood-fed females of Anopheles farauti, Anopheles bancroftii, Anopheles longirostris, Anopheles sundaicus, Anopheles vagus, Anopheles kochi, Anopheles annularis, Anopheles tessellatus, Culex vishnui, Culex quinquefasciatus and Mansonia spp were collected while resting on the barrier screens. In addition, female Anopheles punctulatus and Armigeres spp as well as male An. farauti, Cx. vishnui, Cx. quinquefasciatus and Aedes species were similarly captured.ConclusionsBuilding barrier screens as temporary resting sites in areas where mosquitoes were likely to fly was an extremely time-effective method for collecting an unbiased representative sample of engorged mosquitoes for determining the human blood index.

【 授权许可】

CC BY   
© Burkot et al.; licensee BioMed Central Ltd. 2013