DipM is required for peptidoglycan hydrolysis during chloroplast division

BMC Plant Biology

Research Article

Yukihiro Kabeya¹  Takayuki Fujiwara¹  Shin-ya Miyagishima¹  Mamoru Sugita²  Chieko Sugita² 

[1] Center for Frontier Research, National Institute of Genetics, 1111 Yata, 411-8540, Mishima, Shizuoka, Japan;Center for Gene Research, Nagoya University, 464-8602, Chikusa-ku, Nagoya, Japan;

关键词: Chloroplast division;    DipM;    Endosymbiosis;    Glaucophytes;    Peptidoglycan;   

DOI  :  10.1186/1471-2229-14-57

 received in 2013-12-31, accepted in 2014-02-26,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundChloroplasts have evolved from a cyanobacterial endosymbiont and their continuity has been maintained over time by chloroplast division, a process which is performed by the constriction of a ring-like division complex at the division site. The division complex has retained certain components of the cyanobacterial division complex, which function inside the chloroplast. It also contains components developed by the host cell, which function outside of the chloroplast and are believed to generate constrictive force from the cytosolic side, at least in red algae and Viridiplantae. In contrast to the chloroplasts in these lineages, those in glaucophyte algae possess a peptidoglycan layer between the two envelope membranes, as do cyanobacteria.ResultsIn this study, we show that chloroplast division in the glaucophyte C. paradoxa does not involve any known chloroplast division proteins of the host eukaryotic origin, but rather, peptidoglycan spitting and probably the outer envelope division process rely on peptidoglycan hydrolyzing activity at the division site by the DipM protein, as in cyanobacterial cell division. In addition, we found that DipM is required for normal chloroplast division in the moss Physcomitrella patens.ConclusionsThese results suggest that the regulation of peptidoglycan splitting was essential for chloroplast division in the early evolution of chloroplasts and this activity is likely still involved in chloroplast division in Viridiplantae.

【 授权许可】

CC BY   
© Miyagishima et al.; licensee BioMed Central Ltd. 2014