PCR-diagnosis of Anaplasma marginale in cattle populations of Ecuador and its molecular identification through sequencing of ribosomal 16S fragments

BMC Veterinary Research

Research Article

Jorge Ron-Román¹  Leandro Tana-Hernández²  Katherine Navarrete-Arroyo²  María Augusta Chávez-Larrea³  Armando Reyna-Bello⁴ 

[1] Grupo de Investigación en Sanidad Animal y Humana (GISAH), Carrera Ingeniería Agropecuaria, Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador;Grupo de Investigación en Sanidad Animal y Humana (GISAH), Carrera Ingeniería en Biotecnología, Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador;Grupo de Investigación en Sanidad Animal y Humana (GISAH), Carrera Ingeniería en Biotecnología, Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador;Carrera de Ingeniería en Biotecnología, Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas – ESPE, P.O.BOX: 171-5-231, Av. General Rumiñahui s/n, Sangolquí, Ecuador;Grupo de Investigación en Sanidad Animal y Humana (GISAH), Carrera Ingeniería en Biotecnología, Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador;Investigador-Prometeo, Departamento de Ciencias de la Vida, Carrera Ingeniería en Biotecnología, Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador;

关键词: Bovine Anaplasmosis;    Anaplasma marginale;    msp5;    16S rRNA;    Bovine disease;    Ecuador;   

DOI  :  10.1186/s12917-017-1311-1

 received in 2017-04-28, accepted in 2017-11-28,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBovine anaplasmosis is an endemic disease in tropical and subtropical areas. It is caused by a bacterium named Anaplasma marginale, and represents an economic problem for cattle farmers due to the losses it generates, such as: mortalities, reduced production, quarantine measures, treatments and control of vectors. The method most often used to diagnose this haemotrophic bacterium is direct examination on blood smear, which sensitivity and specificity are limited compared to other methods such as PCR. The present study aimed at investigating the presence of A. marginale in dairy cattle of Luz de América commune, province of Santo Domingo de los Tsachilas. Two PCRs were used to amplify specific regions of the Rickettsia for its molecular identification.ResultsAt first, 151 blood samples were tested: msp5 specific gene of A. marginale was identified in 130 samples, meaning 86.1% of them were infected by the rickettsia. Two positive samples were further randomly selected to confirm the presence of A. marginale through amplification, cloning and sequencing of the conserved region of gene 16S rRNA. The analysis of sequences obtained through cloning revealed a 100% identity between both samples and those registered in GenBank for A. marginale.ConclusionThis is the first report and molecular identification of A. marginale in the bovine population of Ecuador and its prevalence was high at the level of farms and animals. These results demonstrate the importance of proceeding to evaluate and characterize bovine Anaplasmosis in Ecuador in order to establish control measures and reduce their impact.

【 授权许可】

CC BY   
© The Author(s). 2017